Anti-AZI2 Rabbit Monoclonal Antibody for WB and ELISA - Q9H6S1

Core Product Specifications and Parameters

Biological Background: AZI2 Function and Localization

• AZI2 (5-azacytidine-induced protein 2), also known as NAP1 (Nak-associated protein 1) or TILP, is a 44935 Da adapter protein encoded by the AZI2 gene.
• Functions as an adapter that binds the serine/threonine-protein kinase TBK1 and IKBKE, activating TBK1 and facilitating its oligomerization. Related references: PMID:14560022 PMID:21931631
• Enhances TBK1-mediated phosphorylation of NF-kappa-B p65 subunit RELA and promotes NF-kappa-B activation induced by TBK1, TNF, or PMA. Related references: PMID:14560022 PMID:21931631
• Participates in antiviral innate immunity by contributing to IFNB promoter activation via TICAM1. Related references: PMID:15611223
• Localizes predominantly to the cytoplasm.
• Widely expressed across tissues, with abundant levels observed in the pancreas and testis. Related references: PMID:14560022
• Undergoes alternative splicing and is subject to phosphorylation and ubiquitin-like conjugation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal region (aa 282-387) of human AZI2; consider this when designing detection antibodies for sandwich ELISA.
• For Western blotting, a band at approximately 45 kDa is expected; validate in your sample system, noting that AZI2 is widely expressed.
• Given cross-reactivity with mouse and rat, this antibody may be suitable for studies in these species; however, confirm reactivity with your specific model.
• ELISA applications can be explored; optimal antibody concentration and buffer conditions should be determined experimentally.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For researchers studying innate immunity and NF-κB signaling, we supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and sourcing of rare-target raw materials. Partner with CamelBio to streamline your IVD assay development from project initiation to market-ready kits.