Anti-Axl Monoclonal Antibody for WB - P30530

Core Product Specifications and Parameters

Biological Background: Axl Function and Localization

• Axl, also known as tyrosine-protein kinase receptor UFO or AXL oncogene, is a receptor tyrosine kinase that binds the growth factor GAS6, initiating signal transduction pathways that regulate cell survival, proliferation, migration, and differentiation.
• Upon ligand binding, Axl dimerizes and autophosphorylates, leading to recruitment and tyrosine phosphorylation of downstream effectors including PI3K regulatory subunits (PIK3R1, PIK3R2, PIK3R3), GRB2, PLCG1, LCK, and PTPN11, ultimately activating the AKT signaling pathway.
• GAS6/AXL signaling supports diverse physiological processes such as endothelial cell survival during acidification, optimal natural killer cell development, hepatic regeneration, gonadotropin-releasing hormone neuron survival and migration, platelet activation, and regulation of thrombotic responses.
• Axl negatively regulates Toll-like receptor (TLR)-mediated innate immune responses, contributing to immune modulation.
• The protein acts as a host cell receptor for several viruses, including Lassa virus, lymphocytic choriomeningitis virus, and Ebolavirus, likely via GAS6-mediated recognition of phosphatidylserine on the viral envelope.
• Axl facilitates Zika virus entry into glial cells, Sertoli cells, and astrocytes; the virus potentiates Axl kinase activity to antagonize type I interferon signaling through an SOCS1-dependent mechanism. Related references: PMID:28076778, PMID:29379210, PMID:31311882
• Subcellularly, Axl is localized to the cell membrane, consistent with its role as a transmembrane receptor.
• Tissue expression profiling shows high Axl levels in metastatic colon tumors, moderate expression in primary colon tumors, and weak expression in normal colon tissue, highlighting its association with cancer progression.

Experimental Guidance and Technical Tips

• The immunogen spans the extracellular domain (aa 33–449) of human Axl, suggesting potential for native receptor recognition in cell-based applications; verify performance in your specific assay setup.
• For Western blot, predicted molecular weight is ~98 kDa; consider using metastatic colon cancer cell lines (e.g., HCT116, HT-29) as positive controls due to their high Axl expression.
• When developing ELISA assays, explore pairing with a validated detection antibody for sandwich format; the antibody’s cross-reactivity with mouse Axl may enable preclinical studies in murine models.
• Confirm optimal antibody dilution, blocking, and washing conditions empirically, as results can vary with sample preparation and detection systems.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop sourcing partner for IVD raw materials, offering comprehensive antibody solutions including validated pairs and optimized monoclonals like this anti-Axl reagent. With expertise in oncology and cell signaling targets, we support your diagnostic development from concept to clinic with bulk reagents, custom sourcing, and technical consultation.