Anti-AXIN2 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9Y2T1

Core Product Specifications and Parameters

Biological Background: AXIN2 Function and Localization

• AXIN2 (Axin-2), also known as Axil, Axis inhibition protein 2, or Conductin, is a key negative regulator of the canonical Wnt signaling pathway.
• It functions by down-regulating β-catenin levels, likely through facilitating the phosphorylation of β-catenin and APC by GSK3B, thereby targeting β-catenin for degradation.
• The protein is predominantly localized in the cytoplasm, where it exerts its inhibitory effect on Wnt signal transduction.
• According to UniProt, AXIN2 is expressed in brain and lymphoblast tissues, suggesting tissue-specific roles in development and immune function.
• AXIN2 is classified as a developmental protein and is implicated in key cellular processes, with reported post-translational modifications including phosphorylation and ADP-ribosylation.
• The UniProt record for AXIN2 (Q9Y2T1) includes keywords such as Ubl conjugation and reference proteome, indicating its involvement in ubiquitin-like protein modification and its status as a well-characterized protein.
• The protein has a calculated molecular weight of approximately 93.6 kDa (UniProt) or 94 kDa as measured.

Experimental Guidance and Technical Tips

• The immunogen sequence (aa 743-843) lies within the C-terminal region of AXIN2, which may influence antibody specificity; consider validating with full-length protein or known interaction partners.
• For Western blot (WB), a band around 94 kDa is expected; using appropriate positive controls from brain or lymphoblast tissues may aid detection.
• For IHC-P and IF/ICC, cytoplasmic staining is anticipated given the subcellular localization of AXIN2; include negative controls (e.g., omission of primary antibody) to confirm specificity.
• ELISA applications may benefit from optimizing coating antigen and antibody concentration; the recombinant immunogen could serve as a standard.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive IVD raw-material solutions, including this anti-AXIN2 polyclonal antibody, ideal for Wnt signaling research. With validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents, we support diagnostic manufacturers and research laboratories from concept to clinic. Contact us for rare-target sourcing and custom technical services.