Anti-AXIN2 Polyclonal Antibody for WB, ELISA - Q9Y2T1

Core Product Specifications and Parameters

Biological Background: AXIN2 Function and Localization

• AXIN2, also known as Axin-2, Axin-like protein (Axil), Axis inhibition protein 2, or Conductin, is a key negative regulator of the canonical Wnt signaling pathway.
• It functions by promoting the phosphorylation and subsequent degradation of beta-catenin, likely through facilitating the phosphorylation of beta-catenin and the tumor suppressor APC by glycogen synthase kinase 3 beta (GSK3B).
• As a scaffold protein, AXIN2 assembles the beta-catenin destruction complex, thereby tightly controlling beta-catenin levels and downstream Wnt target gene expression.
• The protein is predominantly localized in the cytoplasm, consistent with its role in signal transduction and protein complex assembly.
• AXIN2 is expressed in specific tissues, notably the brain and lymphoblast cells, suggesting roles in both neural development and immune function.
• The protein undergoes post-translational modifications including ADP-ribosylation, phosphorylation, and ubiquitination, which may regulate its stability and activity.
• As a developmental protein involved in cell fate decisions and tissue patterning, AXIN2 is often studied in the context of cancer, embryonic development, and stem cell biology.

Experimental Guidance and Technical Tips

• The immunogen used for this antibody is a synthetic peptide from amino acids 400–500 of human AXIN2. This region may be selected to avoid homology with other axin family members; however, antibody specificity should be confirmed in the relevant cell or tissue system.
• In western blot (WB), a band at approximately 94 kDa is expected. Consider using appropriate positive controls such as cell lines or tissues known to express AXIN2 (e.g., brain or lymphoblast derived cells).
• This antibody demonstrates cross-reactivity with mouse and rat, making it suitable for studies using these common model organisms. Validate regional-specific expression patterns in the tissue or cell type of interest.
• For ELISA, titration of both coating antigen and detection antibody is recommended to achieve optimal signal-to-noise ratios. Consider pairing with a validated detection antibody if developing a sandwich ELISA.
• Given the role of AXIN2 as a negative feedback target of Wnt signaling, its expression may increase upon pathway activation. Thus, treat with Wnt ligands or GSK3 inhibitors to upregulate AXIN2 levels for easier detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to premium IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. Whether you need ready-to-use AXIN2 antibodies for Wnt pathway research or require custom sourcing of rare-target reagents, our comprehensive supply chain and technical support bridge the gap from concept to clinic. Partner with CamelBio to accelerate your assay development with reliable, high-quality immunoreagents.