Anti-AVPR2 Rabbit Polyclonal Antibody for WB, ELISA - P30518

Core Product Specifications and Parameters

Biological Background: AVPR2 Function and Localization

• The vasopressin V2 receptor (AVPR2), also known as the antidiuretic hormone receptor, is a G protein-coupled receptor that mediates arginine vasopressin signaling, primarily promoting renal water reabsorption and activating adenylate cyclase via G(s) proteins. Related references: PMID:1534149, PMID:19440390, PMID:33664408, PMID:33742150
• The receptor undergoes alternative splicing, giving rise to multiple isoforms.
• AVPR2 is localized to the cell membrane, where it functions as a transmembrane transducer.
• The protein is subject to post-translational modifications including glycosylation, disulfide bond formation, phosphorylation, and palmitoylation, which regulate receptor activity and trafficking.
• AVPR2 is predominantly expressed in the kidney, consistent with its role in antidiuretic hormone signaling and water homeostasis.
• Mutations in AVPR2 are associated with nephrogenic diabetes insipidus, a disease characterized by impaired renal water reabsorption.

Experimental Guidance and Technical Tips

• Given the immunogen design encompassing residues 232-371, the antibody may detect both native and denatured AVPR2 in WB; consider optimizing reducing conditions for clear bands around 40 kDa.
• For ELISA applications, recombinant protein or cell lysates expressing AVPR2 can be used as coating antigen; validate cross-reactivity with endogenous proteins in the relevant sample system.
• Confirm specificity in human kidney tissue or cell lines (e.g., HEK293 transfected with AVPR2) and include appropriate controls.
• Due to the receptor's glycosylation and lipidation, band shifts or multiple bands may occur; use peptide blocking or knockout validation to confirm identity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with streamlined access to IVD raw materials for GPCR and renal disease studies. Beyond this AVPR2 antibody, we offer validated antibody pairs, optimized recombinant proteins, and bulk ancillary reagents to support your assay development. Rely on CamelBio as your trusted partner for rare target material sourcing and one-stop procurement from concept to clinic.