Anti-AVPR1A Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P37288

Core Product Specifications and Parameters

Biological Background: AVPR1A Function and Localization

• AVPR1A (Vasopressin V1a receptor) is a G protein-coupled receptor (GPCR) that serves as the receptor for arginine vasopressin, a neuropeptide hormone.
• This receptor activates the phosphatidylinositol-calcium second messenger system via G proteins, leading to downstream signaling cascades.
• AVPR1A has been implicated in social behaviors, such as affiliation and attachment, particularly in animal models.
• The protein is localized predominantly to the cell membrane, as a multi-pass transmembrane receptor.
• It is subject to post-translational modifications including glycosylation, palmitoylation, and phosphorylation, which may regulate its function and trafficking.
• Structurally, AVPR1A contains seven transmembrane helices, disulfide bonds, and a palmitoylated cysteine residue, typical of class A GPCRs.
• The gene AVPR1A encodes the receptor, and alternative names include AVPR V1a and antidiuretic hormone receptor 1a.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal intracellular domain (aa 349-418), which is unique to AVPR1A and may reduce cross-reactivity with other vasopressin receptor subtypes (V1b, V2).
• For Western blotting, detect a band at approximately 47 kDa; however, glycosylation and other modifications may result in a slightly higher apparent molecular weight.
• In immunofluorescence, permeabilization is required to access the intracellular epitope; validate subcellular localization in known AVPR1A-expressing cells.
• This polyclonal antibody may be used for ELISA as a capture or detection reagent; titer and specificity should be empirically determined.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, monoclonal and polyclonal antibodies, and bulk ancillary reagents. For GPCR targets like AVPR1A, we offer customized sourcing solutions to support your assay development from concept to clinic, ensuring a reliable supply chain for social behavior and cardiovascular research. Contact us to streamline your procurement and focus on innovation.