Anti-AVP Polyclonal Antibody for WB, ELISA - P01185

Core Product Specifications and Parameters

Biological Background: AVP Function and Localization

• The human AVP gene encodes a preprohormone that is proteolytically processed into the antidiuretic hormone arginine vasopressin, its carrier protein neurophysin 2, and a C-terminal glycopeptide copeptin.
• Arginine vasopressin binds to vasopressin receptors (V1aR, V1bR, V2R) to exert direct antidiuretic effects on the kidney and vasoconstriction of peripheral vessels, playing a central role in fluid homeostasis and cardiovascular regulation.
• Neurophysin 2 acts as a specific carrier, binding vasopressin in the hypothalamus and transporting it along axons to the posterior pituitary for stimulated secretion, thereby protecting the hormone from degradation.
• The precursor protein localizes predominantly to cytoplasmic secretory vesicles, from where mature peptides are secreted into the circulation upon appropriate physiological signals.
• Post-translational modifications include amidation of the vasopressin peptide, formation of a disulfide bond essential for biological activity, and cleavage at paired basic residues to generate the mature peptides.
• Keywords associated with AVP include Diabetes insipidus, highlighting its pathogenic relevance; the hormone causes water retention, and defects lead to excessive dilute urine production.

Experimental Guidance and Technical Tips

• The immunogen covers the majority of the preprohormone (aa 20-164), excluding the signal peptide; the antibody may recognize both precursor and processed forms, with the precursor migrating near the indicated 17 kDa and smaller mature peptides potentially visible depending on gel conditions.
• For Western blotting, consider using hypothalamus or pituitary lysates as positive control tissues; reduce and denature samples thoroughly to disrupt disulfide-linked oligomers.
• Cross-reactivity with mouse and rat has been reported; validate species-specific detection by comparing known expression profiles in target tissues.
• In ELISA applications, ensure the recombinant immunogen is used as a standard for quantification, and account for possible matrix effects with serum or plasma samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic developers and researchers with a streamlined supply chain for IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. This anti-AVP polyclonal antibody supports neuroendocrine, fluid balance, and cardiovascular research. Leverage our rare target sourcing and technical consulting to accelerate your assay development from concept to clinic.