Anti-Aurora B Rabbit Polyclonal Antibody for WB, ELISA - Q96GD4

Core Product Specifications and Parameters

Biological Background: Aurora B Kinase Function and Localization

• Aurora B (AURKB) is a serine/threonine-protein kinase and an integral component of the chromosomal passenger complex (CPC), a master regulator of mitosis. Related references: PMID:11516652, PMID:12925766, PMID:14610074

• The CPC localizes to centromeres, ensuring proper chromosome alignment and segregation, and promotes spindle microtubule stabilization and assembly. Related references: PMID:11516652, PMID:12925766, PMID:14610074

• AURKB controls the bipolar attachment of spindle microtubules to kinetochores and triggers the onset of cytokinesis. Related references: PMID:15249581

• It is essential for the assembly of the central spindle and cleavage furrow, completing cell division. Related references: PMID:12458200, PMID:12686604

• Well-characterized substrates include histone H3 (phosphorylated at Ser10 and Ser28), INCENP, BIRC5, and other CPC components, as well as p53, vimentin, and CGAS. Related references: PMID:11784863, PMID:11856369, PMID:20959462, PMID:33542149

• Subcellularly, Aurora B dynamically localizes to the nucleus, chromosome centromeres, kinetochores, mitotic spindle, and midbody.

• It is highly expressed in thymus, spleen, testis, and fetal liver, upregulated in many cancer cells, and notably high in metastatic liver but absent in normal liver.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 1–100 of human Aurora B (NP_004208.2); for Western blot, expect a band around 39 kDa and consider validating in mitotically synchronized cell lines.
• For ELISA, optimize coating antigen concentration and include appropriate peptide or recombinant protein controls; cross-reactivity with mouse and rat supports rodent model studies but validate in target species.
• This polyclonal antibody may detect multiple isoforms or post-translational modifications; confirm specificity using relevant controls and consider peptide blocking where needed.

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