Anti-Aurora B Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - Q96GD4

Core Product Specifications and Parameters

Biological Background: Aurora B Function and Localization

• Aurora B (Aurora kinase B) is a serine/threonine-protein kinase and a core component of the chromosomal passenger complex (CPC), which is essential for proper chromosome alignment, segregation, and cytokinesis during mitosis. Related references: PMID:11516652 PMID:12925766 PMID:14610074

• It facilitates the bipolar attachment of spindle microtubules to kinetochores, ensuring accurate chromosome segregation. Related references: PMID:15249581

• Aurora B is required for central spindle assembly and cleavage furrow formation, playing a critical role in the onset of cytokinesis. Related references: PMID:12458200 PMID:12686604

• It acts as a key component of the cytokinesis checkpoint, phosphorylating CHMP4C to delay abscission until chromosome bridges are resolved, thereby preventing DNA damage. Related references: PMID:22422861 PMID:24814515

• Aurora B phosphorylates several CPC subunits and downstream substrates, including INCENP (enhancing its own activity), BIRC5/survivin, CDCA8/borealin, and histone H3 at Ser10 and Ser28 (H3S10ph and H3S28ph), which are marks of mitotic chromatin. Related references: PMID:11516652 PMID:11784863 PMID:11856369

• During the G2-M transition, Aurora B phosphorylates CGAS, inhibiting its liquid phase separation and activation to prevent autoimmunity. Related references: PMID:33542149

• Aurora B is highly expressed in thymus, spleen, lung, testis, colon, placenta, and fetal liver, and its expression is upregulated in cancer cells during M phase.

• Subcellularly, it localizes to the nucleus, chromosomes, centromeres, kinetochores, spindle, and midbody, consistent with its mitotic functions.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to the N-terminal region (aa 1–100) of human Aurora B. For Western blot, expect a band at approximately 39 kDa; consider using mitotic cell lysates as positive controls to enhance detection.
• In immunofluorescence/ICC applications, Aurora B typically localizes to centromeres, the spindle midzone, and midbody; appropriate fixation and permeabilization methods should be validated for your cell type.
• For ELISA, the antibody may be used as a capture or detection reagent; pair with a suitable detection antibody and validate with recombinant Aurora B protein.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop platform for IVD raw material sourcing, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. Specializing in cell cycle and mitosis targets like Aurora B, we bridge the gap between research and diagnostic development, providing reliable access to high-quality reagents. Contact our team for tailored support in sourcing rare-target raw materials to accelerate your project from concept to clinic.