Anti-Aurora B Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q96GD4

Core Product Specifications and Parameters

Biological Background: Aurora B Function and Localization

• Aurora B (AURKB) is a serine/threonine-protein kinase and a core component of the chromosomal passenger complex (CPC), essential for proper chromosome segregation and cytokinesis. Related references: PMID:11516652, PMID:12925766, PMID:14610074
• It localizes to the centromere and spindle midzone, regulating kinetochore-microtubule attachments, spindle assembly, and cleavage furrow formation during mitosis. Related references: PMID:15249581, PMID:12458200, PMID:12686604
• AURKB is a key component of the cytokinesis checkpoint, delaying abscission to prevent chromosome bridge resolution defects and DNA damage by phosphorylating CHMP4C and retaining VPS4 at the midbody. Related references: PMID:22422861, PMID:24814515
• It phosphorylates multiple substrates including histones H3 (Ser10 and Ser28), INCENP, BIRC5, CDCA8, CENPA, vimentin, and p53, impacting chromatin condensation, transcription regulation, and cytoskeletal dynamics. Related references: PMID:11756469, PMID:11784863, PMID:11856369, PMID:20959462
• Subcellularly, Aurora B is found at the nucleus, chromosome centromeres, kinetochores, mitotic spindle, and midbody during cell division.
• Highly expressed in thymus, and also present in spleen, lung, testis, colon, placenta, and fetal liver; expression is cell cycle-dependent and upregulated in cancer cells.
• The kinase activity is regulated by acetylation, phosphorylation, and ubiquitination, with key roles in mitosis, cell cycle, and cancer.

Experimental Guidance and Technical Tips

• This monoclonal antibody was raised against a recombinant fusion protein containing amino acids 1–100 of human TLR4; validate specificity for Aurora B in your target samples and applications.
• For Western blotting (WB), a major band at ~39 kDa is expected corresponding to Aurora B; use lysates from mitotically enriched cells for stronger signal.
• In immunohistochemistry (IHC-P), antigen retrieval (e.g., citrate buffer pH 6.0) and testing in tissues with a high proliferative index (e.g., thymus, tumors) are recommended.
• For immunofluorescence (IF/ICC), co-staining with mitotic markers (e.g., phospho-H3 Ser10, α-tubulin) can help confirm spindle or midbody localization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive range of IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and ancillary reagents for diagnostic assay development. Our Aurora B antibody supports research in cell cycle regulation, mitosis, and oncology, and we provide one-stop sourcing from concept to clinic. Explore our portfolio for high-quality antibodies and tailored technical services to accelerate your diagnostic projects.