Anti-AURKC Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q9UQB9

Core Product Specifications and Parameters

Biological Background: AURKC Function and Localization

• AURKC, also known as Aurora kinase C, AIE2, AIK3, ARK3, and STK13, is a serine/threonine-protein kinase that belongs to the Aurora kinase family, which includes AURKA and AURKB.
• AURKC is a component of the chromosomal passenger complex (CPC), a key regulator of mitosis, essential for correct chromosome alignment, segregation, chromatin-induced microtubule stabilization, and spindle assembly.
• AURKC plays a critical role in meiosis and spermatogenesis; its expression is predominantly detected in testis, with maximum levels during M phase.
• AURKC phosphorylates histone H3 at 'Ser-10' and 'Ser-28', a function redundant with AURKB; it can rescue an AURKB knockdown, suggesting overlapping roles in cell division.
• AURKC phosphorylates CPC subunits BIRC5/survivin and INCENP, leading to enhanced kinase activity, and also phosphorylates TACC1 at 'Ser-228', linking it to cell division regulation.
• Subcellularly, AURKC localizes to the nucleus, chromosome centromeres, and the cytoplasmic mitotic spindle, consistent with its mitotic functions.
• The protein has a predicted molecular weight of ~35.6 kDa (UniProt) and an apparent weight of 36 kDa (experimental), and is subject to alternative splicing producing two isoforms expressed in testis.
• Although primarily expressed in testis, elevated AURKC levels are observed in a subset of cancer cell lines such as Hep-G2, Huh-7, and HeLa, indicating a possible role in oncogenesis.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-100) of human AURKC; the antibody may recognize both isoforms of AURKC and potentially cross-react with AURKB due to sequence homology in this region. Verify specificity in the relevant sample system, especially when using knockdown controls or isoform-specific detection is required.
• For Western blotting, a band at ~36 kDa is expected; consider using reducing conditions and appropriate protein loading controls. Optimize antibody dilution and detection conditions for your specific cell or tissue lysates.
• In immunohistochemistry (paraffin), antigen retrieval may be necessary; validate staining in positive controls such as testis or mitotic cancer cell lines.
• For ELISA, pair the antibody with a suitable capture/detection system and validate linearity and sensitivity for the target antigen; note that cross-reactivity to related Aurora kinases should be assessed.
• Given the expression pattern, consider using cell cycle-synchronized samples to enrich for AURKC signal, as expression peaks in M phase.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a dedicated IVD raw-material sourcing partner, providing validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw materials across the entire diagnostic development pipeline. For cell division and cancer biomarker research, this AURKC antibody supports investigations into chromosomal passenger complex functions and can be integrated into assay development workflows. CamelBio’s comprehensive portfolio and technical consulting bridge the gap from concept to clinic, offering a single point of contact for all raw material needs.