Anti-AURKA Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - O14965

Core Product Specifications and Parameters

Biological Background: AURKA Function and Localization

• AURKA (Aurora kinase A) is a mitotic serine/threonine kinase critical for cell cycle progression, mitotic spindle assembly, centrosome maturation, chromosomal alignment, and cytokinesis.
  Related references: PMID:11039908, PMID:12390251, PMID:17125279, PMID:14523000, PMID:26246606
• The protein localizes predominantly to the centrosome, spindle poles, and centrioles; additional pools are found at the basal body, cilia, and neuronal projections.
• Key substrates include PLK1, TPX2, p53/TP53, HDAC6, and KIF2A, reflecting its roles in spindle regulation, chromosome segregation, and microtubule dynamics.
  Related references: PMID:11551964, PMID:14702041, PMID:15128871, PMID:17604723, PMID:20643351, PMID:19351716
• AURKA phosphorylates and destabilizes p53/TP53, thereby modulating the p53 pathway and contributing to oncogenic transformation.
  Related references: PMID:14702041
• It regulates cilia dynamics by phosphorylating HDAC6, promoting cilia disassembly and inhibiting ciliogenesis.
  Related references: PMID:17604723, PMID:20643351
• Highly expressed in testis and various cancer cell lines (e.g., colon, ovarian, prostate, breast, cervical), consistent with its role as a proto-oncogene.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 301-403 of human AURKA (C-terminal region), which may detect full-length and truncated variants; verify in the relevant sample system.
• For Western blotting, an ~46 kDa band is expected; use lysates from cells with known AURKA expression (e.g., HeLa, cancer lines) as positive controls.
• In immunofluorescence/ICC, optimize fixation and permeabilization to preserve centrosomal and spindle staining.
• For ELISA, consider coating with the immunogen peptide or recombinant AURKA protein to assess antibody reactivity.
• Validate cross-reactivity with mouse samples as indicated, but be aware of potential species-specific differences.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement for diagnostic manufacturers and research labs, offering a comprehensive portfolio of validated antibodies, antigen pairs, and ancillary reagents. This anti-AURKA polyclonal antibody supports cancer and cell cycle research applications, complementing our expanding catalog of high-quality antibodies and bulk raw materials. Contact us to explore tailored sourcing solutions for your project needs.