Anti-AUH Rabbit Polyclonal Antibody for WB, ELISA - Q13825

Core Product Specifications and Parameters

Biological Background: AUH Function and Localization

• AUH encodes methylglutaconyl-CoA hydratase, a mitochondrial enzyme also known as AU-specific RNA-binding enoyl-CoA hydratase. It plays a dual role in leucine catabolism and RNA metabolism.
• Catalyzes the hydration of 3-methylglutaconyl-CoA to 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) in the leucine degradation pathway, a key step for ketone body production (PubMed:11738050, 12434311, 12655555, 16640564).
  Related references:
  PMID:11738050 PMID:12434311 PMID:12655555 PMID:16640564
• Exhibits broader substrate specificity in vitro, including glutaconyl-CoA, crotonyl-CoA, and 3-methylcrotonyl-CoA, albeit with lower affinity (PubMed:16640564).
  Related references:
  PMID:16640564
• Binds to AU-rich elements (AREs) in mRNA, suggesting a role in regulating mRNA stability and decay (PubMed:7892223).
  Related references:
  PMID:7892223
• May function as an itaconyl-CoA hydratase in the C5-dicarboxylate catabolism pathway, detoxifying the antimicrobial metabolite itaconate produced by macrophages (PubMed:29056341).
  Related references:
  PMID:29056341
• Localizes predominantly to the mitochondrial matrix, consistent with its metabolic functions.
• Post-translational modifications include acetylation, and the protein undergoes alternative splicing, adding functional diversity.
• Genetic variants of AUH are associated with disease, highlighting its clinical importance.

Experimental Guidance and Technical Tips

• The immunogen used corresponds to amino acids 68–339 of human AUH, covering a large portion of the mature protein. This may favor recognition of both native and denatured conformations in various applications.
• For Western blotting, a band around 36 kDa (or 35.6 kDa as per UniProt) is expected. Use mitochondrial-enriched lysates or cell lines with confirmed AUH expression as positive controls to verify band specificity.
• In ELISA, direct coating with recombinant AUH protein or the immunogen fragment can serve as a capture strategy. Consider pairing with a detection antibody recognizing a distinct epitope for sandwich ELISA development.
• To confirm target specificity, siRNA-mediated knockdown or CRISPR knockout of AUH in relevant cell lines is recommended to observe loss of signal in WB.
• Given the high sequence homology among human, mouse, and rat AUH, the antibody may cross-react with these species. Always validate reactivity in the appropriate species-derived samples if working with non-human systems.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive range of IVD raw materials and sourcing solutions for diagnostic and research communities, covering key metabolic targets like AUH. Our portfolio includes validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and customized sourcing for rare targets. For researchers developing assays targeting mitochondrial metabolism or RNA-binding proteins, we provide reliable access to high-quality reagents and technical consultation.