Anti-AUH Monoclonal Antibody for WB and ELISA - Q13825

Core Product Specifications and Parameters

Biological Background: AUH Function and Localization

• AUH, also known as methylglutaconyl-CoA hydratase, catalyzes the reversible hydration of 3-methylglutaconyl-CoA to 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA), the fifth step in leucine degradation. Related references: PMID:11738050 PMID:12434311 PMID:12655555
• AUH can catalyze the reverse reaction and also processes alternative substrates including glutaconyl-CoA, 3-methylcrotonyl-CoA, crotonyl-CoA, and 3-hydroxybutanoyl-CoA, though with lower efficiency. Related references: PMID:16640564
• Originally identified as an AU-rich element (ARE)-binding protein, AUH binds to AREs in vitro, suggesting a role in mRNA stability regulation. Related references: PMID:7892223
• AUH may function as an itaconyl-CoA hydratase, converting itaconyl-CoA to citramalyl-CoA in the C5-dicarboxylate catabolism pathway, contributing to itaconate detoxification in macrophages. Related references: PMID:29056341
• The protein localizes to the mitochondrion, consistent with its role in branched-chain amino acid catabolism.
• Post-translational modifications include acetylation, and alternative splicing generates multiple isoforms.
• Mutations in the AUH gene are linked to 3-methylglutaconic aciduria, a metabolic disorder.
• The protein has a known 3D structure, classifying it as a lyase.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 68-339, covering the mature mitochondrial protein; consider using positive controls expressing AUH such as liver or kidney tissues.
• For Western blotting, a band near 36 kDa is expected; validate detection in the relevant species and cell line.
• In IF/ICC or IHC-P, mitochondrial localization can be confirmed by co-staining with mitochondrial markers.
• Cross-reactivity with mouse and rat is indicated; validation in these species is recommended.
• ELISA protocols may require optimization of coating antigen and antibody concentration given the monoclonal nature.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with a one-stop sourcing platform for IVD raw materials, including validated monoclonal antibodies like AUH Rabbit mAb for metabolism and mitochondrial research. Our supply capabilities extend to optimized antibody pairs, bulk ancillary reagents, and rare target sourcing, supporting your workflow from concept to clinic.