Anti-ATXN2 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q99700

Core Product Specifications and Parameters

Biological Background: ATXN2 Function and Localization

• ATXN2 encodes ataxin-2, also known as spinocerebellar ataxia type 2 protein and trinucleotide repeat-containing gene 13 protein.
• The primary function of ATXN2 involves negative regulation of endocytic EGFR internalization at the plasma membrane, influencing receptor trafficking.
• Ataxin-2 localizes to the cytoplasm, consistent with its role in intracellular trafficking and signaling.
• Tissue expression is broad, with highest levels in brain. Isoform 1 predominates in the brain and spinal cord, while isoform 4 is more abundant in the cerebellum. Within the brain, it is detected in the amygdala, caudate nucleus, corpus callosum, hippocampus, hypothalamus, substantia nigra, subthalamic nucleus, and thalamus.
• ATXN2 is subject to phosphorylation, ubiquitination, and methylation, and its dysfunction via CAG trinucleotide repeat expansion is linked to spinocerebellar ataxia type 2, amyotrophic lateral sclerosis, and Parkinsonism.
• Alternative splicing generates multiple isoforms, which may exhibit tissue-specific expression patterns.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 1250–1331 of human ATXN2. Verify target detection across relevant isoforms in your sample system.
• For Western blotting, ATXN2 has a predicted molecular weight of ~140 kDa; consider using a 6–8% SDS-PAGE gel and optimized transfer conditions for high-molecular-weight proteins.
• In immunohistochemistry (IHC-P), validate the antibody in brain tissue sections where ATXN2 is known to be highly expressed. Include appropriate negative controls.
• For immunofluorescence/ICC, expect a cytoplasmic staining pattern. Co-staining with organelle markers may help confirm localization.
• Cross-reactivity with mouse and rat allows application in rodent models; confirm performance in tissue or cell lysates from these species.
• ELISA applications may require careful optimization of coating antigen and detection conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to high-quality IVD raw materials. This anti-ATXN2 polyclonal antibody supports research in neurodegeneration and EGFR biology, and is one of many optimized antibody products we offer. Whether you need validated antibody pairs, bulk ancillary reagents, or assistance sourcing rare targets, CamelBio is your reliable partner from concept to clinic.