Anti-ATXN10 Polyclonal Antibody for WB, IF/ICC, ELISA - Q9UBB4

Core Product Specifications and Parameters

Biological Background: ATXN10 Function and Localization

• Ataxin-10 (ATXN10) is also known as brain protein E46 homolog and spinocerebellar ataxia type 10 protein.
• ATXN10 may play a role in the regulation of cytokinesis, as indicated by functional studies. Related references: PMID:21857149 PMID:25666058
• It may also play a role in signaling by stimulating protein glycosylation and induces neuritogenesis by activating the Ras-MAP kinase pathway, which is necessary for the survival of cerebellar neurons (By similarity).
• ATXN10 is expressed in the central nervous system.
• Subcellular localization includes the cytoplasm, perinuclear region, midbody, centrosome, centriole, and cilium basal body.
• Key post-translational modifications include phosphorylation, methylation, and Ubl conjugation. It is also subject to alternative splicing.
• Mutations in ATXN10 are associated with spinocerebellar ataxia type 10, a neurodegenerative disorder.

Experimental Guidance and Technical Tips

• For Western blot, a band at approximately 53 kDa is expected; consider using a neuronal cell line like SH-SY5Y as a positive control.
• In immunofluorescence/ICC, expect cytoplasmic staining with possible enrichment at the perinuclear region, midbody, and centrosome; permeabilization is required.
• When performing ELISA, titrate the antibody to determine the optimal working concentration for your specific assay format.
• Validate the antibody in your relevant sample system to confirm reactivity, as cross-reactivity with mouse and rat has been reported.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibody pairs, optimized polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. For research into ATXN10, which is implicated in neurodegeneration and cytokinesis, we can supply the necessary reagents and support from concept to clinic.