Anti-ATRX Rabbit Polyclonal Antibody for WB, ELISA - P46100

Core Product Specifications and Parameters

Biological Background: ATRX Function and Localization

• ATRX (Transcriptional regulator ATRX, X-linked helicase II, XNP, Znf-HX) is a chromatin remodeler encoded by the ATRX gene (RAD54L, XH2) in humans.
• Plays a central role in transcriptional regulation and chromatin remodeling; facilitates DNA replication at specific genomic loci and binds DNA tandem repeats and G4 quadruplex structures, incorporating H3.3-containing nucleosomes to stabilize these regions.
• Functions as the catalytic subunit of the ATRX:DAXX complex, which exhibits ATP-dependent DNA translocase activity and mediates replication-independent deposition of histone H3.3 at pericentric repeats and telomeres.
• Conflicting evidence exists regarding its role in TERRA regulation: one study indicates it is not sufficient to increase TERRA expression or decrease telomeric chromatin condensation in fibroblasts. Related references: PMID:24500201
• ATRX forms a complex with ZNF274, TRIM28, and SETDB1 to deposit and maintain H3K9me3 at the 3' exons of zinc-finger genes. Related references: PMID:27029610
• Acts as a negative regulator of histone macroH2A1 incorporation at telomeres and the alpha-globin cluster.
• Participates in allele-specific gene expression at the imprinted IGF2/H19 locus, facilitating chromatin occupancy of SMC1 and CTCF on the maternal allele.
• Subcellular localization: predominantly nuclear, associated with chromosomes, telomeres, and PML bodies.
• Tissue specificity: ubiquitously expressed in human tissues.

Experimental Guidance and Technical Tips

• The immunogen used is a synthetic peptide corresponding to amino acids 2100–2200 of human ATRX. Consider verifying antibody specificity using peptide competition assays or by comparing signals in ATRX knockout versus wild-type samples.
• For Western blotting, note that ATRX is a large protein (~283 kDa); use a low-percentage polyacrylamide gel (e.g., 6–8%) and ensure efficient transfer by extending transfer times or using optimized protocols for high-molecular-weight proteins.
• This antibody detects both human and mouse ATRX; if working with mouse tissues, validate cross-reactivity in your model system.
• In ELISA applications, the immunogen peptide can be employed as a coating antigen to determine antibody titer or to set up competitive assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with comprehensive access to IVD raw materials, including validated antibodies for critical targets such as ATRX, a key chromatin remodeler with implications in cancer, developmental disorders, and epigenetic regulation. Whether you need matched antibody pairs, bulk polyclonal or monoclonal antibodies, or ancillary reagents, CamelBio streamlines procurement and supports your assay development pipeline from concept to clinic. For rare or custom targets like ATRX, our one-stop sourcing services ensure supply continuity and technical alignment.