Anti-ATRIP Polyclonal Antibody for WB, ELISA - Q8WXE1

Core Product Specifications and Parameters

Biological Background: ATRIP Function and Localization

• ATRIP (ATR-interacting protein, also designated ATM and Rad3-related-interacting protein) is a critical mediator of the DNA damage checkpoint.
• It is required for ATR expression, likely by stabilizing the ATR kinase, and thus essential for ATR-mediated checkpoint signaling.
• ATRIP is a nuclear protein involved in DNA repair processes and the cellular response to genotoxic stress.
• The protein is ubiquitously expressed, consistent with its fundamental role in genome maintenance.
• Alternative splicing generates multiple isoforms, and the protein features a coiled-coil domain.
• It is subject to phosphorylation, a modification that may regulate its function in DNA damage pathways.
• Key associated keywords from UniProt include DNA damage, DNA repair, nucleus, phosphoprotein, and coiled coil.

Experimental Guidance and Technical Tips

• The immunogen region (aa 1-260) targets the N-terminus, which may detect full-length ATRIP and certain isoforms; verify band patterns in your sample system.
• For Western blot, consider using nuclear-enriched fractions from human or mouse cell lines; induction of DNA damage (e.g., UV, etoposide) may reveal functional modifications.
• The antibody shows cross-reactivity with mouse, but validation in your specific mouse strain/tissue is recommended.
• For ELISA applications, direct coating with the recombinant immunogen or capture in sandwich format can be explored; optimize conditions empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop provider for IVD raw materials, delivering validated antibody pairs, polyclonal and monoclonal antibodies, ancillary reagents, and custom sourcing of rare targets. Our ATRIP antibody supports researchers and diagnostic developers focusing on DNA damage response and genomic stability, from early-stage assay design to clinical application.