Anti-ATRIP Monoclonal Antibody for WB, ELISA - Q8WXE1

Core Product Specifications and Parameters

Biological Background: ATRIP Function and Localization

• ATRIP is an ATR-interacting protein essential for DNA damage checkpoint signaling. It is required for ATR expression, likely by stabilizing the protein.
• It plays a critical role in DNA repair and maintaining genomic integrity upon replication stress.
• ATRIP is localized to the nucleus, where it functions in the DNA damage response.
• The protein is ubiquitously expressed across human tissues.
• It undergoes phosphorylation, which may regulate its activity in damage signaling.
• Alternative splicing produces multiple isoforms, potentially with distinct functions.
• Structural features include a coiled-coil domain, and it is classified under DNA damage, DNA repair, and phosphoprotein keywords.

Experimental Guidance and Technical Tips

• The immunogen corresponds to an internal peptide (aa 200-300), so the antibody should recognize native and denatured ATRIP; consider this when designing assays.
• For Western blot (WB), an expected band near 86 kDa should be observed; validate detection in relevant cell lines expressing ATRIP.
• For ELISA, the antibody can be used as a capture or detection reagent; optimize coating concentrations and pairing with a detection antibody if needed.
• Cross-reactivity with mouse and rat ATRIP is reported, making it suitable for studies across these species; confirm reactivity in your model system.
• Although not listed, the antibody may work in immunohistochemistry or immunofluorescence; pilot testing with appropriate controls is recommended if these applications are desired.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For DNA damage and repair research, we offer validated antibody pairs, optimized monoclonal antibodies like this ATRIP clone, and bulk ancillary reagents to support your assay development. Our sourcing expertise also extends to rare and emerging targets, ensuring your project moves rapidly from benchtop to validation.