Anti-ATR Rabbit Monoclonal Antibody for WB and ELISA - Q13535

Core Product Specifications and Parameters

Biological Background: ATR Function and Localization

• Serine/threonine protein kinase activating checkpoint signaling upon genotoxic stresses such as ionizing radiation, UV, or DNA replication stalling, functioning as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Related references: PMID:10597277, PMID:10608806, PMID:10859164

• Phosphorylates multiple downstream targets including BRCA1, CHEK1, MCM2, RAD17, RBBP8, RPA2, SMC1, and p53/TP53, collectively inhibiting DNA replication and mitosis while promoting DNA repair, recombination, and apoptosis. Related references: PMID:11114888, PMID:11418864, PMID:11865061

• Phosphorylates 'Ser-139' of histone variant H2AX at DNA damage sites, regulating the DNA damage response. Related references: PMID:11673449

• Critical for maintaining fragile site stability and efficient regulation of centrosome duplication. Related references: PMID:12526805

• Acts as a regulator of the S-G2 transition by restricting CDK1 activity during S-phase to prevent premature entry into G2. Related references: PMID:30139873

• Functions as a mechanical stress sensor and regulator of nuclear envelope integrity; relocalizes to the nuclear envelope upon mechanical stress and mediates a checkpoint via CHEK1 phosphorylation. Also promotes nuclear envelope rupture in response to DNA damage by phosphorylating LMNA at 'Ser-282', triggering lamin disassembly. Related references: PMID:25083873, PMID:37788673, PMID:37832547

• Involved in innate immunity by localizing to micronuclei from genome instability, phosphorylating LMNA at 'Ser-395' (priming further CDK1 phosphorylation), and promoting micronuclei envelope rupture, which activates the cGAS-STING pathway and type I interferon response. Related references: PMID:37788673

• Subcellular localization: Nucleus, Chromosome, Nuclear envelope. Tissue specificity: Ubiquitous, with highest expression in testis. Isoform 2 is found in pancreas, placenta and liver but not in heart, testis and ovary.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 2100-2200 of human ATR. This region may influence antibody specificity; verify the antibody’s performance in your target application.
• For Western Blot, expect a band at ~301 kDa. Consider using low-percentage SDS-PAGE gels (e.g., 6%) and extended transfer times to ensure efficient protein transfer.
• The antibody has been reported for use in WB and ELISA and shows cross-reactivity with human and mouse ATR. Validate in the relevant species and tissue type, such as testis where ATR expression is highest.
• For ELISA, optimize coating and detection conditions using standard protocols.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your one-stop sourcing bridge for IVD raw materials, providing validated antibodies and reagents for DNA damage and cell cycle research. This ATR rabbit monoclonal antibody is one of many high-quality immunological tools we supply to support diagnostic and research applications. Backed by comprehensive technical support and bulk procurement options, we help accelerate your assay development from concept to clinic.