Anti-ATPIF1 Rabbit Polyclonal Antibody (KO Validated) for WB and IP - Q9UII2

Core Product Specifications and Parameters

Biological Background: ATPIF1 Function and Localization

• ATPIF1 (ATPase inhibitor, mitochondrial) is a nuclear-encoded protein that localizes to the mitochondrion and functions as a key regulator of ATP synthase activity.
• Acts as an endogenous inhibitor of the F1Fo-ATP synthase to limit ATP depletion when the mitochondrial membrane potential falls, preventing the enzyme from hydrolyzing ATP (PubMed:35926043). Related references: PMID:35926043
• Indirectly regulates heme synthesis in erythroid tissues by modulating mitochondrial pH and redox potential, which facilitates the incorporation of iron into protoporphyrin IX by ferrochelatase (FECH).
• The mature protein (after mitochondrial targeting peptide cleavage) has a molecular weight of approximately 12.2 kDa and contains a coiled-coil domain.
• Post-translational modifications include phosphorylation, which may modulate its inhibitory activity.
• Alternative splicing produces multiple isoforms, suggesting tissue-specific or condition-specific regulation.

Experimental Guidance and Technical Tips

• The immunogen used for this polyclonal antibody corresponds to amino acids 26-106 of human ATPIF1, covering the mature protein region after removal of the mitochondrial transit peptide. This design ensures recognition of the processed, active form.
• The antibody shows cross-reactivity with mouse ATPIF1 as per supplier data; however, validation in the specific sample system is recommended before large-scale use.
• For Western blotting and immunoprecipitation, consider using standard protocols with appropriate positive and negative controls. Due to the polyclonal nature, background may vary with sample type and dilution.
• For immunofluorescence, mitochondrial staining is expected. Co-staining with mitochondrial markers (e.g., MitoTracker or Tom20) can help confirm specificity.
• Note that as a polyclonal antibody, lot-to-lot consistency may vary; each new lot should be tested in pilot experiments to verify performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, including this anti-ATPIF1 polyclonal antibody. Whether you need validated antibody pairs for mitochondrial research, bulk ancillary reagents, or assistance sourcing rare targets, our one-stop supply chain can streamline your development workflow. From concept to clinic, CamelBio is dedicated to providing consistent quality and reliable access to the critical reagents your assays require.