Anti-ATPB Rabbit Polyclonal Antibody - P06576

Core Product Specifications and Parameters

Biological Background: ATPB Function and Localization

• The ATP synthase F(1) complex subunit beta, also known as ATP5F1B, ATP5B, ATPMB, ATPSB, is a catalytic core subunit of mitochondrial ATP synthase (Complex V).
• It synthesizes ATP from ADP using the proton gradient across the inner mitochondrial membrane, working in conjunction with subunit alpha (ATP5F1A) in the F(1) domain. Related references: PMID:37244256
• Localizes to the mitochondrial inner membrane.
• Under physiological conditions, it functions in ATP synthesis, although its ATP hydrolase activity can be activated artificially in vitro.
• Subject to post-translational modifications including acetylation, phosphorylation, and glycosylation, as indicated by proteomics studies.
• Genetic mutations in this protein are associated with dystonia and other mitochondrial disorders.
• Plays a key role in cellular energy metabolism and is a central component of the oxidative phosphorylation pathway.

Experimental Guidance and Technical Tips

• For western blot, a band around 57 kDa is expected in human, mouse, and rat samples; reducing conditions are recommended.
• In immunohistochemistry (IHC-P), pre-validate antigen retrieval methods, as the target is a mitochondrial inner membrane protein.
• In immunofluorescence/immunocytochemistry (IF/ICC), co-stain with mitochondrial markers to confirm localization.
• ELISA performance may vary; cross-reactivity with other ATP synthase subunit beta homologs should be assessed.
• As a polyclonal antibody, lot-to-lot consistency should be verified for critical quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop sourcing partner for IVD raw materials, offering this Anti-ATPB rabbit polyclonal antibody to support research and diagnostic development in mitochondrial biology and energy metabolism. Our supply network covers validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents, plus flexible sourcing for rare targets. This streamlines your project from feasibility to final product, ensuring reliable access to the materials you need.