Anti-ATPB Mouse Monoclonal Antibody for WB, IHC-P, ELISA - P06576

Core Product Specifications and Parameters

Biological Background: ATPB Function and Localization

• ATP synthase F1 beta subunit (ATP5B) is the catalytic component of the mitochondrial ATP synthase complex (Complex V) that synthesizes ATP from ADP using the proton motive force.
• Together with subunit alpha (ATP5F1A), it forms the F1 catalytic core responsible for ATP production. Related references: PMID:37244256
• ATP synthesis is coupled to proton translocation through a rotary mechanism of the central stalk.
• Located in the mitochondrial inner membrane.
• Under physiological conditions, the enzyme functions as an ATP synthase; ATP hydrolase activity can be artificially induced in vitro.
• The protein contains nucleotide-binding domains and requires magnesium ions for activity.
• Post-translational modifications include acetylation and phosphorylation, which may regulate its function.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide derived from human ATPB; verify sequence homology with target species to ensure expected cross-reactivity.
• For Western blotting, the predicted molecular weight is ~57 kDa (UniProt: 56.6 kDa); validate using appropriate positive control lysates.
• For IHC-P and IF/ICC, consider antigen retrieval conditions and optimize primary antibody concentration.
• This antibody cross-reacts with mouse and rat, making it suitable for mammalian model studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides one-stop access to high-quality IVD raw materials, including validated monoclonal antibodies like this anti-ATP5B clone. Our portfolio supports mitochondrial research, metabolic disorders, and diagnostic assay development. With bulk supply, custom antibody production, and expert consulting, we help streamline your project from concept to clinic.