Anti-ATP8B3 Rabbit Polyclonal Antibody for WB and ELISA - O60423

Core Product Specifications and Parameters

Biological Background: ATP8B3 Function and Localization

• ATP8B3, also known as ATPase class I type 8B member 3 or ATPIK, is a member of the P4-ATPase flippase family that catalyzes ATP-dependent translocation of aminophospholipids from the exoplasmic to the cytoplasmic leaflet of cellular membranes.
• The primary function of ATP8B3 is to maintain the asymmetric distribution of phosphatidylserine (PS) in membranes, a process critical for membrane curvature, vesicle formation, and lipid signaling.
• In spermatozoa, ATP8B3 plays a key role in acrosome reactions and the binding of sperm to the zona pellucida, indicating its importance in fertilization.
• The protein localizes to the acrosome membrane (a specialized secretory vesicle) and the endoplasmic reticulum membrane, consistent with its role in phospholipid transport.
• Isoform 3 of ATP8B3 exhibits testis-specific expression, highlighting its specialized function in male reproductive tissues.
• ATP8B3 is a multi-pass transmembrane protein with nucleotide-binding, ATP-binding, and metal-binding domains, classifying it as a translocase involved in lipid transport.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 600–900 of human ATP8B3 (NP_620168.1); the antibody may recognize epitopes within this region. Validate positive reactivity in your target species and sample type.
• For Western blot (WB), a band near 147 kDa is predicted based on the full-length protein molecular weight. Optimize blocking and detection conditions for this high-molecular-weight target.
• This polyclonal antibody has been tested in ELISA; consider using it as a capture or detection reagent, and confirm appropriate coating concentrations and detection systems for your assay format.
• Given the testis-specific expression of isoform 3, protein lysates from human testis or spermatozoa are recommended for initial WB characterization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides IVD manufacturers, clinical laboratories, and research institutes with streamlined access to critical raw materials like this Anti-ATP8B3 antibody. As a one-stop sourcing partner, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and efficient procurement of rare-target raw materials to accelerate your reproductive biology and fertility diagnostics development. Let us bridge your supply needs from concept to clinic.