Anti-ATP8 Monoclonal Antibody for WB, IP, IHC-P, ELISA - P03928

Core Product Specifications and Parameters

Biological Background: ATP8 Function and Localization

• ATP8, also known as ATPase8, MTATP8, or A6L, is the mitochondrial F-ATP synthase subunit 8 encoded by the mitochondrial gene MT-ATP8.
• It is a core component of the membrane F(0) domain of the ATP synthase complex (Complex V), where it contributes to the proton channel essential for ATP synthesis. Related references: PMID:37244256
• ATP synthase couples the proton gradient generated by the electron transport chain to the synthesis of ATP from ADP, utilizing a rotary mechanism. Related references: PMID:37244256
• The complex consists of a soluble F(1) catalytic head and a membrane-embedded F(0) domain linked by central and peripheral stalks; ATP8 is part of the F(0) domain. Related references: PMID:37244256
• Subcellularly, ATP8 localizes to the mitochondrial membrane.
• Key post-translational modifications include acetylation, and the protein is associated with keywords such as cardiomyopathy and primary mitochondrial disease, indicating its clinical relevance.
• The protein has a molecular weight of approximately 8 kDa (UniProt: 7992 Da).

Experimental Guidance and Technical Tips

• For Western blot, consider standard denaturing conditions and detect at approximately 8 kDa; validate in human and rat samples.
• For immunoprecipitation, ensure mitochondrial lysis conditions are optimized.
• For immunohistochemistry, optimize antigen retrieval and validate in relevant tissue sections; positive controls may include heart or skeletal muscle.
• ELISA applications may benefit from pairing with a suitable capture antibody and validating in the target sample matrix.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, including validated monoclonal antibodies for ATP synthase subunits like ATP8. Our comprehensive supply capabilities encompass bulk ancillary reagents, optimized antibody pairs, and rare target sourcing to support your assay development from concept to clinic. We are committed to being your reliable sourcing partner for high-quality mitochondrial research and diagnostic reagents.