Anti-ATP7A Rabbit Polyclonal Antibody for WB and ELISA - Q04656

Core Product Specifications and Parameters

Biological Background: ATP7A Function and Localization

• ATP7A, also known as Copper pump 1 or Menkes disease-associated protein, is a copper-transporting ATPase 1 encoded by the ATP7A gene in humans.
• It functions as an ATP-driven copper (Cu⁺) pump crucial for intracellular copper ion homeostasis, acquiring Cu⁺ from cytoplasmic donors and delivering it across membranes. Related references: PMID:10419525 PMID:11092760 PMID:28389643
• The catalytic cycle couples ATP hydrolysis to copper translocation, involving transient phosphorylation and a conformational switch from inward-facing to outward-facing state. Related references: PMID:10419525 PMID:19453293 PMID:19917612
• Under low cytosolic copper, ATP7A localizes to the trans-Golgi network to supply Cu⁺ to secretory pathway cuproenzymes; upon copper elevation, it relocalizes to the plasma membrane to export excess copper. Related references: PMID:11092760 PMID:28389643 PMID:10419525
• In neurons, ATP7A regulates copper efflux and synaptic transmission, and supplies Cu⁺ to enzymes like PAM, TYR, and SOD3, playing a dual role in neuronal function and survival. Related references: PMID:28389643
• In melanosomes, it provides copper cofactor to tyrosinase (TYR) for melanin biosynthesis.
• ATP7A is widely expressed, including in heart, brain, lung, muscle, kidney, and pancreas, and at lower levels in placenta; it is also found in fibroblasts and vascular endothelial cells.
• The protein undergoes alternative splicing and is subject to post-translational modifications such as phosphorylation and glycosylation, with roles in various cellular compartments including Golgi, membrane, endosomes, and synapses.

Experimental Guidance and Technical Tips

• This antibody is generated against a recombinant fusion protein corresponding to the N-terminal region (amino acids 1-79) of human ATP7A. Consider verifying specificity by Western blot analysis using cell lines or tissue lysates known to express ATP7A.
• For Western blot (WB), ATP7A is predicted to run at approximately 163 kDa; however, the product specification notes a wide range of 11-172 kDa, possibly reflecting isoforms or degradation products. Optimize gel percentage and transfer conditions accordingly, and include appropriate protease inhibitors.
• Cross-reactivity with rat ATP7A has been reported; validate performance in rat samples if cross-species detection is desired.
• For ELISA applications, consider testing a range of antibody dilutions and employ appropriate positive and negative controls.
• Since ATP7A is a transmembrane protein, the choice of lysis buffer (e.g., RIPA) and sample preparation (with or without heating) may influence detection. Empirically determine optimal conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing bridge for IVD raw materials, providing diagnostic manufacturers and researchers with high-quality reagents and technical support. This anti-ATP7A antibody supports investigations into copper homeostasis and Menkes disease pathology. We offer validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents to streamline your assay development and rare target sourcing needs.