Anti-ATP7A Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - Q04656

Core Product Specifications and Parameters

Biological Background: ATP7A Function and Localization

• ATP7A is a copper-transporting P-type ATPase that couples ATP hydrolysis to the translocation of Cu(+) ions across cellular membranes.
  Related references: PMID:10419525, PMID:11092760, PMID:28389643, PMID:19453293, PMID:19917612
• It plays a central role in intracellular copper homeostasis, shuttling between the trans-Golgi network (for cuproenzyme loading) and the plasma membrane (for copper efflux) in a copper concentration-dependent manner.
  Related references: PMID:11092760, PMID:28389643, PMID:10419525
• In neurons, ATP7A supplies copper to cuproenzymes such as PAM, TYR, and SOD3, and may contribute to synaptic copper regulation.
  Related references: PMID:28389643
• The protein localizes to multiple cellular compartments including the trans-Golgi network, plasma membrane, endosomes, melanosomes, and neuronal projections, reflecting its dynamic trafficking.
• Mutations in the ATP7A gene cause Menkes disease (MNK), a severe X-linked recessive disorder characterized by copper deficiency and neurodegeneration.
• ATP7A is widely expressed, with high transcript and protein levels detected in the heart, brain, lung, muscle, kidney, and pancreas.
  Related references: PMID:8490646, PMID:8490659; at protein level: PMID:16371425

Experimental Guidance and Technical Tips

• For Western blotting, a band corresponding to full-length ATP7A is expected at approximately 163 kDa, but additional bands or a range may be observed due to isoform variations or proteolytic processing; validate with appropriate controls.
• In immunofluorescence (IF/ICC), permeabilization is recommended to allow antibody access to intracellular compartments such as the Golgi and endosomes.
• For ELISA, consider direct or sandwich assay formats and titrate the antibody to achieve optimal signal-to-noise ratios.
• As a rabbit monoclonal antibody, ensure use of compatible anti-rabbit secondary reagents and avoid cross-reactivity by testing in your specific model system.
• Since the immunogen targets the N-terminal region (aa 1-79), the antibody may detect only full-length or certain isoforms; confirm reactivity in your samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a premier one-stop provider of IVD raw materials, technical services, and consulting, empowering diagnostic developers from concept to clinic. This anti-ATP7A rabbit monoclonal antibody supports research into copper metabolism and Menkes disease, and CamelBio can supply validated antibody pairs, optimized monoclonals, ancillary reagents, and rare-target sourcing to accelerate your assay development.