Anti-ATP6V1G3 Polyclonal Antibody for WB, IHC-P, ELISA - Q96LB4

Core Product Specifications and Parameters

Biological Background: ATP6V1G3 Function and Localization

• ATP6V1G3 encodes the V-type proton ATPase subunit G 3, a component of the V1 complex of vacuolar H+-ATPase (V-ATPase), a multi-subunit enzyme responsible for acidifying intracellular compartments and, in some cell types, the extracellular microenvironment.
• The V-ATPase holoenzyme consists of a peripheral V1 sector that hydrolyzes ATP and an integral membrane V0 sector that translocates protons, linking ATP hydrolysis to transmembrane proton pumping.
• ATP6V1G3 is selectively expressed in the kidney, suggesting a specialized role in renal acid secretion and systemic pH regulation.
• Alternative splicing produces multiple transcript variants, which may modulate V-ATPase assembly and activity in different cellular contexts.
• The protein contains a coiled-coil domain, critical for stable interaction within the V1 complex and proper enzyme assembly.
• Key functional annotations include alternative splicing, coiled coil, hydrogen ion transport, ion transport, and proteomics identification, highlighting its transport function and structural motif.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody corresponds to the N-terminal region (amino acids 1–118) of human ATP6V1G3. Consider validating its reactivity with full-length endogenous protein and potential splice isoforms in your model system.
• For Western blotting, a band around 14 kDa is expected. Use appropriate gel percentage (e.g., 12–15% SDS-PAGE) and low-molecular-weight markers to resolve the signal clearly.
• Given kidney-specific expression, immunohistochemical analysis (IHC-P) should include kidney tissue sections as positive controls; ensure proper antigen retrieval and antibody titration.
• Cross-reactivity with mouse is indicated; however, confirm compatibility in your specific mouse strain and tissue type before large-scale studies.
• ELISA applications may benefit from using recombinant ATP6V1G3 protein as a standard; optimize coating and detection conditions for best sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers, laboratories, and research institutes with high-quality IVD raw materials, offering a streamlined path from concept to clinic. This anti-ATP6V1G3 polyclonal antibody supports investigations into renal acidification mechanisms, proton pump biology, and ion transport disorders. Beyond individual antibodies, CamelBio can supply validated antibody pairs, optimized monoclonal/polyclonal reagents, bulk ancillary materials, and assist with sourcing rare-target raw materials to accelerate your assay development and production workflows.