Anti-ATP6V1G2 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - O95670

Core Product Specifications and Parameters

Biological Background: ATP6V1G2 Function and Localization

• ATP6V1G2 (V-type proton ATPase subunit G 2, also known as V-ATPase 13 kDa subunit 2) is a subunit of the V1 domain of vacuolar H+-ATPase (V-ATPase).
• V-ATPase is a multi-subunit enzyme responsible for acidifying intracellular compartments such as lysosomes, endosomes, and secretory vesicles by pumping protons across membranes.
• The V-ATPase also localizes to the plasma membrane in some cell types to acidify the extracellular environment.
• ATP6V1G2 is predominantly expressed in the brain, with evidence of alternative splicing.
• Subcellularly, ATP6V1G2 is found in melanosomes and clathrin-coated vesicle membranes, indicating roles in melanosome physiology and vesicular trafficking.
• The gene belongs to the V-ATPase G subunit family, which includes related proteins involved in proton transport across various tissues.
• Keywords: Alternative splicing, cytoplasmic vesicle, hydrogen ion transport, ion transport, membrane, transport.

Experimental Guidance and Technical Tips

• The immunogen was designed against amino acids 40-100 of human ATP6V1G2; cross-reactivity with mouse and rat orthologs should be validated in the relevant sample system.
• For Western blot (WB), expect a band around 14 kDa; consider using a high-percentage SDS-PAGE gel for optimal separation of low molecular weight proteins.
• Immunohistochemistry (IHC-P) on paraffin-embedded tissues may require antigen retrieval; optimization for brain tissue is recommended given brain-specific expression.
• For ELISA, consider pairing with a suitable detection antibody and validating the assay range with recombinant protein or cell lysates.
• Include appropriate positive and negative controls, such as brain tissue lysates for WB and IHC-P, and known knockout cell lines if available.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, labs, and research institutes with comprehensive access to IVD raw materials, including validated antibody pairs, monoclonal/polyclonal antibodies, and bulk ancillary reagents. This anti-ATP6V1G2 antibody serves as a reliable tool for research into vacuolar proton pump function and intracellular trafficking, with CamelBio's supply chain capable of sourcing rare targets and providing one-stop procurement solutions. For customized antibody development or large-scale orders related to ATP6V1G2 and V-ATPase research, contact our technical team.