Anti-ATP6V1B2 Rabbit Polyclonal Antibody for WB, ELISA - P21281

Core Product Specifications and Parameters

Biological Background: ATP6V1B2 Function and Localization

• ATP6V1B2 encodes the brain isoform of the V-type proton ATPase subunit B, a non-catalytic component of the peripheral V1 complex of vacuolar H+-ATPase (V-ATPase).
• As part of the V-ATPase multisubunit enzyme, ATP6V1B2 contributes to ATP hydrolysis-driven proton translocation, acidifying intracellular compartments such as lysosomes, endosomes, and secretory vesicles (PMID:33065002).
  Related references: PMID:33065002
• The V-ATPase complex acts as an activator for mTORC1 on lysosomal membranes by facilitating the guanine nucleotide exchange factor (GEF) activity of the Ragulator complex, enabling mTORC1 recruitment (PMID:22053050).
  Related references: PMID:22053050
• This subunit is also found at the plasma membrane in some cell types, mediating extracellular acidification; in renal intercalated cells, it can partially compensate for ATP6V1B1 deficiency to secrete protons into urine under baseline conditions (PMID:32001091).
  Related references: PMID:32001091
• Known aliases include ATP6B2, VPP3, VATB, HO57, and Vma2, reflecting its identification across different systems and species.
• Subcellular localization includes apical cell membrane, melanosome, synaptic vesicle membrane, clathrin-coated vesicle membrane, and cytoplasm, consistent with roles in organelle acidification and neurotransmitter loading.
• Tissue expression is prominent in kidney, particularly in the early distal nephron (thick ascending limbs and distal convoluted tubules), highlighting its importance in renal acid-base homeostasis.
• Keywords from UniProt: ATP-binding, deafness, disease variant, hydrogen ion transport, ion transport, membrane, nucleotide-binding, proteomics identification, reference proteome, synapse, transport.

Experimental Guidance and Technical Tips

• The immunogen spans a central region of ATP6V1B2 (amino acids 262-511), which is conserved across human, mouse, and rat; consider this cross-reactivity when designing multi-species experiments.
• For Western blotting, a specific band at approximately 57 kDa is expected; validate detection using lysates from kidney or brain tissues known to express ATP6V1B2.
• ELISA applications may require optimization of coating antigen concentrations and antibody titration; using the recombinant immunogen as a positive control can aid in assay development.
• Given ATP6V1B2’s role in endomembrane acidification, treatments with V-ATPase inhibitors (e.g., bafilomycin A1) could serve as functional controls to assess antibody specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting. This ATP6V1B2 polyclonal antibody supports studies on ion transport, lysosomal biology, and mTORC1 signaling. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to accelerate your R&D and IVD development from concept to clinic.