Anti-ATP6V1B1 Rabbit Polyclonal Antibody for WB, IF-P, ELISA - P15313

Core Product Specifications and Parameters

Biological Background: ATP6V1B1 Function and Localization

• ATP6V1B1 encodes the kidney isoform of the V-ATPase B subunit, also known as the endomembrane proton pump 58 kDa subunit, vacuolar proton pump subunit B1, and previously designated as ATP6B1, VATB, or VPP3.
• As a non-catalytic subunit of the V1 peripheral complex, it is essential for the assembly and activity of the vacuolar-type H+ ATPase, which acidifies intracellular compartments and the extracellular environment (PubMed:16769747, PubMed:32001091). Related references: PMID:16769747 PMID:32001091
• In renal intercalated cells, ATP6V1B1 mediates proton secretion into urine, ensuring correct urinary acidification (PubMed:16769747). Related references: PMID:16769747
• Required for optimal olfactory function by mediating acidification of the nasal olfactory epithelium (By similarity).
• The protein localizes to apical and basolateral cell membranes, consistent with its role in polarized proton transport.
• Primarily expressed in kidney, specifically in the early distal nephron including thick ascending limbs and distal convoluted tubules, and also found in the cochlea and endolymphatic sac (PubMed:16769747, PubMed:29993276, PubMed:9916796). Related references: PMID:16769747 PMID:29993276 PMID:9916796
• Mutations in ATP6V1B1 are associated with sensorineural deafness and distal renal tubular acidosis (DRTA2), highlighting its critical role in hearing and acid–base homeostasis.
• Keywords: ATP-binding, nucleotide-binding, hydrogen ion transport, ion transport, membrane protein – these reflect its core biochemical functions.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (aa 1–280) of human ATP6V1B1; verify that the antibody recognizes full-length endogenous protein in your sample system.
• For Western blot, consider loading 20–30 μg of kidney tissue lysate (human, mouse, or rat) and expect a band near 57 kDa; confirm specificity using a positive control such as transfected cell lysate.
• In immunofluorescence (IF-P), validate staining patterns in kidney sections or cultured renal epithelial cells; apical and basolateral membrane localization should be expected.
• For ELISA applications, optimize coating antigen and detection conditions using recombinant protein or cell lysates; this antibody may capture or detect ATP6V1B1 in sandwich ELISA setups.
• When using mouse or rat samples, cross-reactivity has been confirmed, but always include appropriate species-specific secondary antibodies and controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD antibodies, including this high-quality ATP6V1B1 rabbit polyclonal antibody, to support diagnostic manufacturers and research laboratories in renal disease and deafness studies. Beyond antibodies, we provide validated antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets, ensuring a reliable supply chain from concept to clinic.