Anti-ATP6V0C Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P27449

Core Product Specifications and Parameters

Biological Background: ATP6V0C Function and Localization

• ATP6V0C encodes the V-type proton ATPase 16 kDa proteolipid subunit c, also known as vacuolar proton pump 16 kDa proteolipid subunit c, and aliases include ATPL, VATL, VPPC, Vma3, ATP6C, and ATP6L.
• Functions as the proton-conducting pore-forming subunit of the V0 complex of vacuolar H(+)-ATPase (V-ATPase), a multisubunit enzyme responsible for acidifying intracellular compartments.
  Related references: PMID:33065002, PMID:36074901
• In some cell types, V-ATPase is targeted to the plasma membrane, where it promotes acidification of the extracellular environment (By similarity).
• The V-ATPase complex also acts as an activator for mTORC1 on lysosomal membrane by facilitating the guanine nucleotide exchange factor (GEF) of the Ragulator complex, enabling mTORC1 recruitment.
  Related references: PMID:22053050
• Subcellularly, the protein localizes to membranes of clathrin-coated vesicles, secretory vesicles (e.g., synaptic vesicles), and lysosomes.
• The protein contains four transmembrane helices and is subject to ubiquitin conjugation. It is involved in host-virus interaction, and mutations in the gene are associated with epilepsy.
• The calculated molecular weight of the unprocessed precursor is approximately 15.7 kDa, consistent with the observed ~16 kDa band on Western blots.

Experimental Guidance and Technical Tips

• The antibody was raised against a synthetic peptide spanning amino acids 1-100 of human ATP6V0C. For Western blotting, a band near 16 kDa is anticipated in lysates from Human, Mouse, or Rat cells/tissues.
• In immunofluorescence/immunocytochemistry (IF/ICC), staining of cytoplasmic vesicles and lysosomes is expected. Permeabilization is required for intracellular epitope access; optimize protocol for vesicular compartments.
• For ELISA, the antibody may be used to detect the immunogen peptide or recombinant protein. Validate assay conditions in your experimental system.
• As a polyclonal antibody, lot-to-lot variability may occur. It is advisable to titrate the antibody for each new application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated one-stop partner for IVD raw materials, from discovery to commercialization. This anti-ATP6V0C antibody supports research into lysosomal acidification, mTOR signaling, and V-ATPase-related pathologies. Beyond this polyclonal antibody, CamelBio offers validated matched antibody pairs, recombinant monoclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets—streamlining your assay development and production scaling.