Anti-ATP6AP2 Rabbit Polyclonal Antibody for WB, ELISA - O75787

Core Product Specifications and Parameters

Biological Background: ATP6AP2 (Renin receptor) Function and Localization

• ATP6AP2 encodes the renin receptor, a multifunctional transmembrane protein also known as ATPase H+ transporting lysosomal accessory protein 2, prorenin receptor, and ELDF10, with several alternative splice isoforms reported.
• As a renin and prorenin cellular receptor, it mediates renin-dependent cellular responses by activating the ERK1 and ERK2 signaling pathways. Related references: PMID:12045255
• By increasing the catalytic efficiency of renin in angiotensinogen conversion to angiotensin I, it also plays a role in the renin-angiotensin system (RAS). Related references: PMID:12045255
• It is essential for the assembly of the lysosomal V-type ATPase (V-ATPase) and the acidification of the endo-lysosomal system, crucial for protein degradation and cellular homeostasis. Related references: PMID:29127204, PMID:30374053
• Through its function in V-ATPase assembly and lysosomal acidification, it regulates protein degradation and may control signaling pathways important for proper brain development, synapse morphology, and synaptic transmission.
• The protein localizes to multiple membrane compartments, including the endoplasmic reticulum, lysosomes, endosomes, autophagosomes, clathrin-coated vesicles, synaptic vesicles, and cell projections such as dendritic spines and axons.
• It is widely expressed in brain, heart, placenta, liver, kidney, and pancreas, with specific localization in kidney mesangium, vascular smooth muscles, and placental syncytiotrophoblast cells.
• Mutations in ATP6AP2 are linked to congenital disorder of glycosylation type 2R (CDG2R), X-linked intellectual disability, epilepsy, and Parkinsonism, underscoring its importance in neurodevelopment and metabolic regulation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 251–350) of human ATP6AP2, a sequence well conserved in mouse and rat, consistent with the reported cross-reactivity. Consider verifying sequence homology in your target species if extending beyond the listed reactivity.
• For Western blot, the predicted molecular weight is ~39 kDa (UniProt: 39008 Da). Due to potential post‑translational modifications, the observed band may appear slightly higher. Use appropriate positive controls such as human, mouse, or rat kidney, brain, or placenta lysates, where ATP6AP2 is known to be expressed.
• This polyclonal antibody is suitable for ELISA. As with all polyclonal antibodies, consider potential batch‑to‑batch variability and include relevant controls in each assay.
• Endogenous expression levels can vary between tissues and cell lines; pre‑validation in your specific sample matrix is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a comprehensive one‑stop sourcing platform for IVD raw materials, including validated antibody pairs, monoclonal and polyclonal antibodies, and bulk ancillary reagents. For ATP6AP2‑related studies—spanning the renin‑angiotensin system, lysosomal biology, and neurodevelopmental disorders—CamelBio can assist in sourcing this antibody and offers customized support for rare target raw‑material acquisition, helping you accelerate development from concept to clinic.