Anti-ATP6 Rabbit Monoclonal Antibody for WB, ELISA - P00848

Core Product Specifications and Parameters

Biological Background: ATP6 Function and Localization

• ATP6, also designated F-ATPase protein 6 or ATP synthase F(0) complex subunit a, is an essential component of the mitochondrial ATP synthase (Complex V).
• Together with subunit c, ATP6 forms the proton-conducting channel within the F(0) membrane domain, enabling proton translocation across the inner mitochondrial membrane.
• The rotary mechanism driven by proton flow through ATP6 couples the energy released to ATP synthesis in the F(1) catalytic head domain.
• Two critical half-channels (inlet and outlet) within ATP6 guide protons via a Grotthuss mechanism from the intermembrane space into the matrix.
• ATP6 is a multi-pass transmembrane protein embedded in the mitochondrial inner membrane, encoded by the mitochondrial gene Mtatp6.
• Functional keyword associations include ATP synthesis, hydrogen ion transport, and transmembrane helix, underscoring its role in cellular energy metabolism.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 126–226 of human ATP6, a region potentially conserved across species; verify reactivity in the relevant organism before use.
• For Western blot, the predicted molecular weight is approximately 25 kDa; include appropriate positive and negative controls to confirm band specificity.
• ELISA applications may require optimization of coating antigen concentration, antibody dilution, and blocking conditions to achieve optimal signal-to-noise ratio.
• Given the transmembrane nature of ATP6, denaturing sample preparation (e.g., boiling in Laemmli buffer) is recommended for WB to ensure complete solubilization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal antibodies, and ancillary reagents for mitochondrial and metabolism research applications. Our supply network supports bulk procurement of this anti-ATP6 antibody and related targets, enabling seamless scaling from R&D to clinical manufacturing. Whether you require rare target reagents or tailored solutions, CamelBio bridges your diagnostic development needs.