Anti-ATP5O Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P48047

Core Product Specifications and Parameters

Biological Background: ATP5O Function and Localization

• ATP5O encodes the oligomycin sensitivity conferral protein (OSCP), a stator subunit of the mitochondrial F1F0 ATP synthase (Complex V) that couples proton flow to ATP synthesis (PubMed:37244256). Related references: PMID:37244256
• It forms part of the peripheral stalk, holding the catalytic alpha3beta3 subcomplex and subunit a/ATP6 static relative to the rotary central stalk, enabling efficient energy transfer.
• ATP synthase uses a proton gradient across the inner mitochondrial membrane to drive ADP phosphorylation, producing the majority of cellular ATP.
• Subcellularly, ATP5O is localized exclusively to the mitochondrion inner membrane, consistent with its role in oxidative phosphorylation.
• Post-translational modifications including acetylation and ubiquitination have been identified, potentially influencing complex assembly or stability.
• The protein is highly conserved among human, mouse, and rat, with a predicted molecular weight of approximately 23 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to an internal region (aa 24-213), so the antibody is expected to detect the mature endogenous protein rather than the transit peptide-cleaved form.
• For Western blot (WB), a band near 23 kDa is anticipated; use mitochondrial-enriched lysates for optimal signal.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), verify specificity using appropriate controls, as mitochondrial proteins may show punctate staining.
• Cross-reactivity with mouse and rat has been validated, but confirm performance in your specific assay system.
• For ELISA applications, ensure proper antigen coating and consider using the recombinant immunogen as a positive control.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD raw materials, supporting diagnostic developers with critical reagents for mitochondrial biology and energy metabolism research. In addition to this anti-ATP5O antibody, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. Our portfolio streamlines procurement from concept to clinic, helping you accelerate assay development with reliable, high-quality raw materials.