Anti-ATP5L Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - O75964

Core Product Specifications and Parameters

Biological Background: ATP5L Function and Localization

• ATP5L, also known as ATP5MG or ATP synthase membrane subunit g, is a subunit of the mitochondrial F(0) membrane domain of ATP synthase (Complex V). Related references: PMID:37244256
• The ATP synthase complex harnesses the proton gradient across the inner mitochondrial membrane to drive ATP production from ADP, with the F(0) domain forming the proton channel.
• Subunit g, along with other F(0) components, is essential for the structural integrity and proton translocation activity of Complex V.
• Under physiological conditions, the enzyme functions exclusively as an ATP synthase; artificial conditions in vitro can activate its reverse ATP hydrolase activity.
• The protein localizes to the mitochondrion inner membrane.
• Key post-translational modifications include acetylation, which may regulate enzymatic function or stability.

Experimental Guidance and Technical Tips

• For Western blot (WB), the antibody is expected to detect a band at approximately 11 kDa corresponding to endogenous ATP5L. Validate in your sample system and consider using mitochondrial fractions for enrichment.
• In immunofluorescence/immunocytochemistry (IF/ICC), mitochondrial staining should be observed. Co-localization with known mitochondrial markers is advised to confirm specificity.
• For ELISA, the antibody can be used to detect recombinant or purified ATP5L protein. Optimize coating and detection conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted one-stop IVD raw-material sourcing partner, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents for mitochondrial research. We facilitate seamless procurement of rare-target reagents and provide technical support to bridge your concept-to-clinic development needs.