Anti-ATP5J Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P18859

Core Product Specifications and Parameters

Biological Background: ATP5J Function and Localization

• The ATP5J gene (aliases: ATP5PF, ATP5A, ATPM) encodes the ATP synthase peripheral stalk subunit F6, a critical component of the mitochondrial F1F0 ATP synthase (Complex V) that drives ATP synthesis via proton gradient coupling (PubMed:37244256). Related references: PMID:37244256
• As part of the F0 domain and peripheral stalk, F6 acts as a stator, anchoring the catalytic α3β3 subcomplex and subunit a/ATP6, enabling efficient rotary catalysis.
• The protein localizes exclusively to the inner mitochondrial membrane.
• Post-translational modifications include acetylation and phosphorylation, with evidence for alternative splicing and a mitochondrial transit peptide for proper targeting.
• Keywords associated with this protein include CF(0), hydrogen ion transport, ion transport, and mitochondrion inner membrane, underscoring its role in bioenergetics.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 1-108 of human ATP5J, corresponding to the mature protein after cleavage of the mitochondrial transit peptide. Consider this region when designing epitope-mapping experiments.
• For Western blotting, the predicted molecular weight is approximately 13 kDa; ensure adequate membrane transfer conditions for small proteins.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), since ATP5J localizes to mitochondria, co-staining with mitochondrial markers can confirm specificity.
• When performing ELISA, consider using recombinant ATP5J or mitochondrial extracts as positive controls.
• Cross-reactivity with mouse and rat orthologs has been demonstrated; validate in the relevant sample system to confirm expected band size and localization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive B2B sourcing for IVD raw materials, providing validated antibody pairs, optimized monoclonal/polyclonal antibodies, and ancillary reagents to accelerate diagnostic development. This ATP5J polyclonal antibody exemplifies our capability to supply high-quality tools for mitochondrial and energy metabolism research. For bulk orders or customized sourcing needs, partner with CamelBio to streamline your project from concept to clinic.