Anti-ATP5I Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P56385

Core Product Specifications and Parameters

Biological Background: ATP5I (ATP synthase membrane subunit e) Function and Localization

• ATP5I, also known as ATP5ME (ATP synthase membrane subunit e), is a subunit of the mitochondrial ATP synthase complex (Complex V), specifically part of the F(0) membrane proton channel.
• The ATP synthase complex produces ATP from ADP using a proton gradient generated by the respiratory chain. Subunit e is essential for the complex's structure and function. Related references: PMID:37244256
• The enzyme comprises a soluble F(1) catalytic head and a membrane F(0) proton channel, connected by central and peripheral stalks. Subunit e resides in the F(0) domain.
• Subunit e localizes to the mitochondrion inner membrane, according to UniProt annotations.
• Post-translational modifications include acetylation and phosphorylation, as indicated by UniProt keywords.
• The gene is designated ATP5ME, with aliases ATP5I and ATP5K. The protein has a molecular weight of approximately 8 kDa (7933 Da per UniProt).
• Keywords assigned by UniProt: 3D-structure, CF(0), hydrogen ion transport, ion transport, proteomics identification, and reference proteome.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1–69) of human ATP5I. When performing Western blot, a band around 8 kDa is expected; validate molecular weight markers appropriately.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), consider optimizing antigen retrieval and permeabilization protocols for mitochondrial detection.
• The antibody cross-reacts with mouse and rat, making it suitable for comparative studies across these species; however, verify cross-reactivity in your specific experimental system.
• ELISA and other applications may require titration under your laboratory conditions; include appropriate positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering antibody pairs, bulk ancillary reagents, and custom development services from concept to clinic. This anti-ATP5I polyclonal antibody supports mitochondrial and ATP synthase research, and CamelBio can further assist with sourcing rare-target antibodies, optimized monoclonal/polyclonal reagents, and validated kits to accelerate your diagnostic development.