Anti-ATP5H Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - O75947

Core Product Specifications and Parameters

Biological Background: ATP5H (ATP synthase peripheral stalk subunit d) Function and Localization

• ATP5H encodes subunit d of the mitochondrial ATP synthase (Complex V), which catalyzes ATP synthesis from ADP and inorganic phosphate using the proton gradient generated by the respiratory chain. Related references: PMID:37244256
• Subunit d is an essential component of the peripheral stalk, acting as a stator to hold the catalytic α3β3 subcomplex and subunit a/ATP6 static relative to the rotary central stalk.
• Under physiological conditions, ATP synthase operates exclusively in the ATP synthesis direction; however, its ATP hydrolase activity can be artificially activated in vitro.
• The protein localizes to the mitochondrial inner membrane, consistent with its role in oxidative phosphorylation.
• Post-translational modifications include acetylation, and the gene undergoes alternative splicing, which may generate multiple isoforms.
• High sequence conservation across human, mouse, and rat orthologs supports the reported cross-reactivity of this antibody.

Experimental Guidance and Technical Tips

• For Western blotting, the expected molecular weight is approximately 18 kDa; validate detection in your sample system using a suitable loading control.
• In IHC-P and IF/ICC experiments, antigen retrieval and fixation conditions may influence staining; consider optimizing with known positive control tissues.
• As a polyclonal antibody, lot-to-lot consistency should be verified for critical quantitative assays.
• ELISA applications may require titration of coating antigen and antibody concentrations; confirm specificity with recombinant protein standards.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research labs a streamlined one-stop channel for IVD raw materials, from lead discovery to validated commercial reagents. This anti-ATP5H polyclonal antibody supports mitochondrial research and metabolic disease diagnostics. Beyond this product, we can source validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target raw materials to accelerate your assay development pipelines.