Anti-ATP5G2 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q06055

Core Product Specifications and Parameters

Biological Background: ATP5G2 Function and Localization

• ATP5G2 (gene ATP5MC2) encodes the mitochondrial ATP synthase F(0) complex subunit C2, a proteolipid also known as ATP synthase membrane subunit c locus 2, ATPase protein 9, and ATP synthase lipid-binding protein.
• This subunit is an integral component of the F(0) domain of ATP synthase (Complex V), where it forms a homomeric c-ring of ten subunits that acts as a rotary proton channel, coupling the proton motive force to ATP synthesis.
• As part of the ATP synthase complex, ATP5G2 is essential for mitochondrial oxidative phosphorylation, converting ADP to ATP during cellular respiration.
• The protein localizes to the mitochondrial inner membrane, anchored by transmembrane helices, and contains a mitochondrial transit peptide for import.
• Post-translational modifications include methylation, and it is characterized as a lipid-binding proteolipid critical for membrane integration and function.
• Alternative splicing of the ATP5MC2 gene generates multiple transcript variants, potentially contributing to functional diversity.
• The protein is involved in hydrogen ion transport, a fundamental process in mitochondrial bioenergetics.

Experimental Guidance and Technical Tips

• As a polyclonal antibody raised against a synthetic peptide (aa 50-150), it may recognize multiple epitopes; validate specificity in target species and relevant cell/tissue models.
• For Western blot (WB), expect a band at approximately 15 kDa; optimize sample preparation (e.g., mitochondrial enrichment) and loading controls.
• For immunofluorescence/immunocytochemistry (IF/ICC), mitochondrial staining should be observed; include organelle-specific markers for colocalization confirmation.
• In ELISA applications, the immunizing peptide can be used as a positive control to establish standard curves and assess antibody titer.
• Cross-reactivity has been confirmed for human and mouse; testing in other species (e.g., rat) is recommended if needed.
• The antibody’s performance in detecting multiple isoforms resulting from alternative splicing should be assessed experimentally.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw material sourcing, providing high-quality antibodies like this anti-ATP5G2 polyclonal antibody for mitochondrial bioenergetics research. We supply validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and can source rare targets to support your assay development and manufacturing needs from concept to clinic.