Anti-ATP5G1 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P05496

Core Product Specifications and Parameters

Biological Background: ATP5G1 Function and Localization

• ATP5G1 (also designated ATP5A, ATP5G, or ATP5G1) encodes the mitochondrial ATP synthase F(0) complex subunit C1, a key component of the proton-conducting channel.
• The protein is a subunit of the F(0) membrane domain of ATP synthase (Complex V), which couples proton flow to ATP synthesis.
• Within the F(0) domain, subunit c and subunit a (MT-ATP6) form a proton channel that shuttles protons from the mitochondrial intermembrane space to the matrix, enabling ATP production. Related references: PMID:37244256
• ATP synthase operates via a rotary mechanism, with the F(1) catalytic head and F(0) proton channel connected by central and peripheral stalks. Related references: PMID:37244256
• ATP5G1 localizes to the mitochondrial membrane and is synthesized with an N-terminal transit peptide that directs its import.
• Keywords: Membrane, Mitochondrion, Transmembrane helix, Lipid-binding, Methylation, reflecting its integral membrane association and potential post-translational regulation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 57–136 of human ATP5G1, a region that lies within the mature protein; researchers should verify that this sequence is conserved in their species of interest if using cross-reactive species.
• For Western blot (WB) analysis, a band at approximately 14 kDa is anticipated; however, actual migration may vary based on gel conditions and sample preparation.
• For immunohistochemistry (IHC-P), mitochondrial labeling should be confirmed using appropriate controls, as ATP5G1 is a mitochondrial membrane protein.
• ELISA performance may be influenced by peptide coating conditions; optimization is recommended.
• As the antibody is validated for human, mouse, and rat, cross-reactivity with other species should be tested individually.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated supplier of premium IVD raw materials, connecting diagnostic manufacturers and researchers with validated antibodies, reagents, and technical support from concept to clinic. This ATP5G1 antibody supports mitochondrial bioenergetics research and IVD assay development, backed by CamelBio's comprehensive sourcing expertise and logistics. Whether you need bulk monoclonal antibodies, ancillary reagents, or custom procurement of rare targets, CamelBio streamlines your supply chain for consistent quality.