Anti-ATP5D Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P30049

Core Product Specifications and Parameters

Biological Background: ATP5D Function and Localization

• ATP5D, also designated ATP5F1D, encodes the delta subunit of mitochondrial ATP synthase (Complex V), a key enzyme in oxidative phosphorylation.
• This subunit forms part of the F1 catalytic core's stalk structure, which is essential for coupling proton translocation to ATP synthesis from ADP.
• Together with the gamma subunit (ATP5F1C), ATP5D is indispensable for the biogenesis and proper assembly of the F1 catalytic domain. Related references: PMID:29499186
• The ATP synthase complex's architecture includes a soluble F1 head and membrane-embedded Fo domain, linked by rotating central and stationary peripheral stalks (PubMed:37244256). Related references: PMID:37244256
• ATP5D is localized to the mitochondrial inner membrane.
• Post-translational modifications such as acetylation and the presence of a transit peptide are documented, reflecting its mitochondrial import and regulation.
• The protein is implicated in primary mitochondrial disease, underscoring its clinical relevance in metabolic disorders.

Experimental Guidance and Technical Tips

• The immunogen spans the full-length mature protein (aa 1–168), likely generating antibodies that recognize both native and denatured ATP5D, suitable for WB and IF.
• For WB, expect a band near 17 kDa; validate using mitochondrial-enriched lysates from human, mouse, or rat samples.
• In IF/ICC, co-staining with a mitochondrial marker is advised to confirm subcellular localization.
• ELISA performance should be optimized with recombinant protein or cell lysates; polyclonal nature necessitates lot-specific titration.
• As a polyclonal antibody, consider validating lot-to-lot consistency in critical quantitative applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for high-quality IVD raw materials, supporting diagnostic manufacturers and researchers from early R&D to clinical application. This anti-ATP5D antibody is tailored for mitochondrial research and metabolic disease assay development, backed by CamelBio’s comprehensive portfolio of validated antibody pairs, recombinant proteins, and ancillary reagents. Leverage our streamlined sourcing solutions to accelerate your assay development and secure robust, consistent supply for your diagnostic needs.