Anti-ATP5C1 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P36542

Core Product Specifications and Parameters

Biological Background: ATP5C1 Function and Localization

• ATP5C1 (ATP synthase F1 subunit gamma) is a critical subunit of the mitochondrial F1F0 ATP synthase (Complex V), the enzyme responsible for the synthesis of ATP from ADP and inorganic phosphate using the proton-motive force across the inner mitochondrial membrane. Related references: PMID:37244256
• This protein exists as two major isoforms: Heart isoform, predominantly expressed in cardiac and skeletal muscle, and Liver isoform, expressed in brain, liver, kidney, skin, intestine, stomach, and aorta, reflecting tissue-specific energy demands.
• As the gamma subunit of the central stalk, ATP5C1 couples proton flow-driven rotation in the F0 membrane domain to conformational changes in the F1 catalytic head, enabling ATP synthesis. It also plays an indispensable role in the assembly of the F1 catalytic core, as evidenced by its requirement for the formation of a key F1 assembly intermediate. Related references: PMID:29499186
• ATP5C1 is localized to the mitochondrial inner membrane and contains an N-terminal transit peptide that directs its mitochondrial import. It is subject to post-translational modifications including acetylation and phosphorylation, which may regulate its activity or assembly.
• The predicted molecular weight of ATP5C1 is approximately 33 kDa (32996 Da based on UniProt), which is consistent with the observed band on Western blot.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody corresponds to amino acids 26-298 of human ATP5C1 (NP_001001973.1), which spans the majority of the mature protein after cleavage of the mitochondrial transit peptide; therefore, the antibody is expected to detect the endogenous mature form (approximately 33 kDa) in Western blot.
• For Western blot (WB) applications, consider using mitochondrial-enriched protein lysates from cell lines or tissues (such as heart, skeletal muscle, or liver) that express ATP5C1, and include appropriate molecular weight markers to confirm the 33 kDa band.
• When applying this antibody in immunohistochemistry (IHC) or immunofluorescence (IF), strong signal may be observed in tissues with high mitochondrial content, such as heart and skeletal muscle; co-staining with mitochondrial markers can help verify localization.
• The antibody is reported to react with human, mouse, and rat ATP5C1; however, due to potential sequence differences among species, it is advisable to validate cross-reactivity in the relevant sample system before large-scale experiments.
• For ELISA and IF/ICC applications, it is recommended to perform a titration to determine the optimal working dilution for the specific sample type and experimental conditions.

CamelBio: Your One-Stop Sourcing Bridge

As a specialized supplier for the in vitro diagnostics (IVD) industry, CamelBio streamlines the procurement of high-quality raw materials for diagnostic kit development. The ATP5C1 polyclonal antibody presented here supports research into mitochondrial function, energy metabolism disorders, and related diseases. Beyond antibodies, CamelBio offers validated antibody pairs, bulk ancillary reagents, and customized sourcing for rare targets, enabling diagnostic manufacturers to accelerate their product development from concept to clinical application.