Anti-ATP5A1 Rabbit Monoclonal Antibody for WB, IP, IF, IHC-P, ELISA - P25705

Core Product Specifications and Parameters

Biological Background: ATP5A1 Function and Localization

• ATP5A1 encodes the alpha subunit of mitochondrial ATP synthase (Complex V), which synthesizes ATP from ADP using the proton gradient generated by the electron transport chain. Related references: PMID:37244256
• Together with the catalytic subunit beta (ATP5F1B), it forms the catalytic core of the F1 domain; however, subunit alpha does not bear the catalytic high-affinity ATP-binding sites. Related references: PMID:37244256
• Binds the bacterial siderophore enterobactin and can promote mitochondrial accumulation of enterobactin-derived iron ions. Related references: PMID:30146159
• Located primarily in the mitochondrial inner membrane, but also reported at the cell membrane.
• Expressed in fetal lung, heart, liver, gut, and kidney, with higher levels in fetal brain, retina, and spinal cord.
• Undergoes post-translational modifications including acetylation, glycation, methylation, and phosphorylation.
• Associated with primary mitochondrial disease; mutations have been linked to disorders such as COXPD22.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a central region (aa 200–300) of human ATP5A1, which is highly conserved, likely contributing to the observed cross-reactivity with mouse and rat.
• For Western blotting, a band at approximately 60 kDa is expected; consider using appropriate loading controls and pre-stained markers.
• In immunofluorescence and immunohistochemistry, validate staining patterns in your specific tissue or cell model, as expression can vary. Mitochondrial localization may require counterstaining with organelle markers.
• For immunoprecipitation, titrate the antibody amount to optimize target capture while minimizing background.
• Because ATP5A1 is a mitochondrial protein, sample preparation strategies such as subcellular fractionation may improve detection sensitivity in some applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and researchers with streamlined access to premium IVD raw materials, including this ATP5A1 rabbit monoclonal antibody. For developers building mitochondrial or metabolic assays, CamelBio offers validated antibody pairs, optimized monoclonals, bulk ancillary reagents, and sourcing of rare-target raw materials to accelerate your projects from concept to clinic.