Anti-ATP5A1 Polyclonal Antibody for WB, IF/ICC, ELISA - P25705

Core Product Specifications and Parameters

Biological Background: ATP synthase F(1) complex subunit alpha, mitochondrial

• Core subunit of mitochondrial ATP synthase: ATP5A1 (ATP synthase F1 subunit alpha) is a key component of the F1 catalytic domain of the mitochondrial ATP synthase (Complex V). Together with the beta subunit (ATP5F1B), it forms the catalytic core responsible for ATP production from ADP through a rotary mechanism driven by the proton gradient across the inner mitochondrial membrane.
  Related references:
  PMID:37244256

• Non-catalytic ATP-binding role: Although it binds nucleotides, the alpha subunit does not contain the high-affinity catalytic site for ATP synthesis; instead, it plays a structural and regulatory role in the F1 complex, ensuring proper assembly and function of the catalytic beta subunit.

• Enterobactin binding and iron import: The alpha subunit has been shown to bind the bacterial siderophore enterobactin, facilitating mitochondrial accumulation of enterobactin-derived iron ions, suggesting a role in cellular iron homeostasis.
  Related references:
  PMID:30146159

• Subcellular localization: Primarily located in the mitochondrial inner membrane, with additional detection at the cell membrane. It is a vital component of the oxidative phosphorylation system.

• Tissue expression and disease associations: Expressed broadly, with higher levels in fetal brain, retina, and spinal cord, as well as fetal lung, heart, liver, gut, and kidney. Mutations in ATP5F1A are linked to primary mitochondrial diseases, underscoring its essential role in energy metabolism.

• Post-translational modifications: Subject to acetylation, methylation, and phosphorylation, which may regulate its activity and stability. It is also reported to be glycosylated, reflecting its complex role in mitochondrial function.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1–280 of human ATP5A1, an N-terminal region that is highly conserved across human, mouse, and rat, consistent with the observed cross-reactivity. Researchers may consider using this antibody to detect both endogenous and overexpressed ATP5A1 in mitochondrial extracts.
• For Western blotting (WB), a band around 60 kDa is expected; validate in the relevant species and sample type. For immunofluorescence (IF/ICC) and ELISA, appropriate controls and dilutions should be optimized by the end-user.
• Given ATP5A1's localization to the mitochondrial inner membrane, consider subcellular fractionation or specific staining protocols to enhance detection in imaging applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering one-stop access to validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. For mitochondrial research and energy metabolism studies, CamelBio can supply high-quality antibodies against ATP synthase subunits and related proteins, supporting diagnostic assay development and life science research from concept to clinic.