Anti-ATP4B Rabbit Polyclonal Antibody (Gastric H+/K+ ATPase β subunit) for WB and ELISA - P51164

Core Product Specifications and Parameters

Biological Background: ATP4B Function and Localization

• ATP4B encodes the beta subunit of the gastric H+/K+ ATPase (proton pump), also known as ATP6B, which is primarily expressed in gastric parietal cells.
• This subunit is essential for the structural integrity, membrane targeting, and regulatory function of the H+/K+ ATPase pump.
• The H+/K+ ATPase catalyzes the electroneutral exchange of intracellular H+ for extracellular K+ ions, driving gastric acid secretion with ATP hydrolysis.
• ATP4B stabilizes the outward-facing (E2) conformation of the pump by interacting with the alpha subunit phosphorylation domain, preventing reverse transport.
• It is a single-pass type II transmembrane glycoprotein localized to the apical plasma membrane, containing a signal-anchor sequence.
• Post-translational modifications include N-linked glycosylation and disulfide bond formation, which are critical for its stability and function.
• The protein participates in cell adhesion and ion transport (hydrogen and potassium) as indicated by its keyword annotations.
• ATP4B is a key marker for parietal cell activity and is involved in the maintenance of gastric mucosal barrier function.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 62-291 of ATP4B, which covers a large extracellular/lumenal domain; this region is likely well-presented in native conformations for antibody recognition.
• In Western blot, the predicted molecular weight of unmodified ATP4B is 33 kDa, but glycosylation typically results in a band observed between 60 and 80 kDa under reducing conditions. Use appropriate markers and consider deglycosylation if needed.
• For optimal detection, use freshly prepared or properly stored tissue extracts (e.g., gastric mucosa) to avoid protein degradation.
• This polyclonal antibody is suitable for ELISA; it may also be tested for other applications like immunofluorescence or immunoprecipitation, but such uses require individual validation.
• Cross-reactivity has been demonstrated for human, mouse, and rat; however, sequence homology suggests potential reactivity with other species—verify in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted partner providing comprehensive IVD raw material solutions, from antibody sourcing to technical consulting. This ATP4B rabbit polyclonal antibody complements our portfolio for gastric and ion transport research. Whether you need validated antibody pairs, bulk ancillary reagents, or rare-target raw materials, CamelBio supports your diagnostic and research programs from concept to clinic.