Anti-ATP4B (Hydrogen Potassium ATPase Beta) Rabbit Monoclonal Antibody for IHC-P, ELISA - P51164

Core Product Specifications and Parameters

Biological Background: ATP4B Function and Localization

• Encoded by the ATP4B gene, the protein known as Potassium-transporting ATPase subunit beta (also designated Gastric H(+)/K(+) ATPase subunit beta or Proton pump beta chain) is the regulatory beta subunit of the gastric H⁺/K⁺‑ATPase pump.
• As a core component of the heterodimeric gastric proton pump, it facilitates the electroneutral exchange of luminal K⁺ for cytoplasmic H⁺ across the apical membrane of parietal cells, directly coupling ion translocation to ATP hydrolysis by the catalytic alpha subunit.
• The beta subunit plays essential structural and regulatory roles: it is required for the stable assembly of the alpha/beta complex and for directing the active pump to the apical cell membrane.
• Mechanistically, ATP4B interacts with the phosphorylation domain of the alpha subunit and acts as a ratchet, stabilizing the outward‑facing E2 conformation and preventing the reverse transport reaction.
• Subcellular localization is predominantly at the apical plasma membrane of gastric parietal cells, consistent with its function in acid secretion.
• Key post‑translational features include N‑linked glycosylation and the presence of conserved disulfide bonds, which are critical for proper folding and stability; the mature protein contains a single transmembrane helix and a signal‑anchor sequence.

Experimental Guidance and Technical Tips

• The immunogen was designed as a synthetic peptide spanning residues 192‑291 of human ATP4B, a region predicted to be accessible on the extracellular side of the membrane; this may favor detection of the native protein in IHC‑P.
• For IHC‑P on formalin‑fixed paraffin‑embedded tissues, consider using gastric mucosa sections as a positive control; antigen retrieval optimization may be required.
• In ELISA, the antibody may recognize linear epitopes within the immunogen region; plate‑coating conditions and blocking steps should be tested to minimize background.
• Given the reported cross‑reactivity with mouse and rat, the antibody can be evaluated in rodent gastric tissues, though epitope conservation should be confirmed.
• Experimental conditions should always be validated in the relevant sample system, and appropriate isotype controls should be included to assess assay specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides integrated access to IVD raw materials, supporting diagnostic manufacturers and research laboratories from early‑stage development through clinical application. This ATP4B‑specific antibody aligns with our portfolio for gastric physiology and ion‑transport biomarker studies, offering a validated reagent for IHC‑P and ELISA workflows. Beyond individual antibodies, CamelBio can supply optimized monoclonal and polyclonal antibodies, validated antibody pairs, bulk ancillary reagents, and can assist in sourcing hard‑to‑find targets to accelerate your assay development.