Anti-ATP2B4 Polyclonal Antibody for WB, ELISA - P23634

Core Product Specifications and Parameters

Biological Background: ATP2B4 Function and Localization

• ATP2B4 encodes plasma membrane calcium-transporting ATPase 4 (PMCA4), a magnesium-dependent P-type ATPase that hydrolyzes ATP to drive calcium efflux from the cell, critical for intracellular calcium homeostasis (PubMed:8530416). Related references: PMID:8530416
• Alternative splicing generates multiple isoforms, including the ubiquitously expressed isoform XB, heart-specific isoforms containing segment Z, and isoforms with segment a present in heart, stomach, and brain cortex.
• The protein localizes to the cell membrane and extends into specialized projections such as the cilium and flagellum, where it regulates calcium levels essential for sperm motility (By similarity).
• Calmodulin-binding and phosphorylation events modulate PMCA4 activity, as indicated by the presence of calmodulin-binding and phosphoprotein keywords in curated data.
• Three-dimensional structural studies have resolved the topology of its transmembrane helices and nucleotide-binding domain, informing on its transport mechanism.
• Additionally, the alias matrix-remodeling-associated protein 1 (MXRA1) points to roles in tissue remodeling processes beyond ion transport.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 1141–1205 in the C-terminal region; given PMCA4’s alternative splicing, verify whether this region is present in all target isoforms for your experiment.
• For Western blot, expect a band near 138 kDa; include appropriate molecular weight markers and validate detection in human samples first, then confirm cross-reactivity in mouse tissues if extending studies.
• When performing ELISA, titrate the antibody to achieve optimal signal-to-noise ratios and consider pairing with a monoclonal PMCA4 capture antibody for sandwich ELISA if quantification is needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material partner, streamlining access to validated antibody pairs, optimized polyclonal antibodies like this anti-ATP2B4 pAb, bulk ancillary reagents, and sourcing for rare targets. Whether advancing calcium signaling diagnostics or sperm motility research, our supply chain and technical support bridge the gap from concept to clinic.