Anti-ATP2B2 Rabbit Polyclonal Antibody for WB, ELISA - Q01814

Core Product Specifications and Parameters

Biological Background: ATP2B2 Function and Localization

• ATP2B2 encodes the plasma membrane calcium-transporting ATPase 2 (PMCA2), a P-type ATPase pump that uses ATP hydrolysis to export cytosolic Ca2+ across the plasma membrane, essential for maintaining low intracellular calcium levels. Related references: PMID:15829536, PMID:17234811
• PMCA2 is characterized by fast activation and high Ca2+ clearance rate, making it critical for rapid calcium dynamics in neurons, particularly at the parallel fiber-Purkinje cell synapse where it mediates presynaptic Ca2+ efflux and contributes to long-term depression induction and motor learning.
• In the inner ear, PMCA2 plays an essential role in hearing and balance by shuttling Ca2+ from stereocilia to the endolymph in cochlear hair cells, dissipating mechanotransduction-induced Ca2+ transients, and regulating otoconia formation in the vestibular system. Related references: PMID:15829536, PMID:17234811
• The protein localizes to the plasma membrane, specifically at synapses, as well as apical and basolateral membrane domains in various cell types, consistent with its role in polarized calcium transport.
• Tissue specificity: PMCA2 is predominantly expressed in brain cortex, with lower levels in skeletal muscle, heart, stomach, liver, kidney, and lung. Multiple isoforms exist via alternative splicing, with some containing segments B, X, W, A, or Z, displaying tissue-dependent distribution.
• Key structural and functional motifs include ten transmembrane helices, an ATP-binding domain, a calmodulin-binding domain, and phosphorylation sites; it binds magnesium and calcium ions, and mutations in ATP2B2 are associated with non-syndromic deafness (DFNA82).

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-80) of human ATP2B2, which is likely to recognize multiple isoforms containing this segment. Confirm isoform specificity in your experimental system.
• For Western blot, a band at approximately 137 kDa is expected; consider using protease inhibitors and mild lysis conditions to preserve membrane protein integrity.
• This polyclonal antibody is suitable for ELISA; optimization of coating antigen and antibody dilution may be necessary for the best signal-to-noise ratio.
• Cross-reactivity with mouse and rat is reported, making it suitable for rodent models; validate in the relevant tissue or cell line to confirm reactivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated provider of raw materials for IVD assay development, offering comprehensive support from concept to clinic. This anti-ATP2B2 polyclonal antibody exemplifies our commitment to supplying high-quality reagents for research in calcium signaling and auditory function. Beyond individual antibodies, we deliver validated antibody pairs, optimized monoclonal and polyclonal preparations, bulk ancillary reagents, and customized sourcing for rare or emerging targets to streamline your diagnostic pipeline.