Anti-ATP2B1 Polyclonal Antibody for WB, IF-P, ELISA - P20020

Core Product Specifications and Parameters

Biological Background: ATP2B1 Function and Localization

• ATP2B1 encodes the plasma membrane calcium-transporting ATPase 1 (PMCA1), a member of the P-type ATPase family. It exists in multiple isoforms (B, C, D, K) generated by alternative splicing.
• Catalyzes ATP-driven calcium efflux from the cytoplasm to the extracellular space, crucial for maintaining intracellular calcium homeostasis. Related references: PMID:35358416
• Plays a key role in blood pressure regulation by modulating intracellular calcium and nitric oxide production in vascular smooth muscle cells.
• Positively regulates bone mineralization by promoting intestinal calcium absorption, and has dual roles in osteoclast differentiation and survival (by similarity).
• Modulates insulin sensitivity through calcium/calmodulin-dependent AKT1 and NOS3 activation in endothelial cells. Related references: PMID:29104511
• Localizes to the basolateral cell membrane, synaptic membranes, and synaptic vesicle membranes; involved in synaptic calcium and proton dynamics.
• Tissue-specific expression: isoform B is ubiquitous; isoform C is enriched in brain and muscle; isoform K is found in intestine and liver, with high expression in endometrial epithelial cells. Related references: PMID:21400627
• Keywords: alternative splicing, calmodulin-binding, disease-associated variants (intellectual disability), and multiple transmembrane helices.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide covering the N-terminal region (amino acids 50-150) of human ATP2B1. This region may be conserved across species, consistent with cross-reactivity to mouse and rat.
• For Western blotting (WB), a band at approximately 135 kDa is expected; consider using reducing conditions and validating the detection with appropriate positive controls.
• In immunofluorescence (IF-P), fixation and permeabilization protocols should be empirically optimized for the target cell type and localization (membrane vs. vesicular).
• ELISA applications may require coating with the immunogen peptide or recombinant protein; assay conditions should be standardized in the relevant sample matrix.
• Given the role of PMCA1 in calcium signaling, verify antibody specificity using siRNA knockdown or overexpression models to confirm target identity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your reliable one-stop partner for IVD raw materials, technical services, and consulting from concept to clinic. We offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and sourcing assistance for rare targets. This anti-ATP2B1 polyclonal antibody supports research into calcium transport, cardiovascular function, bone metabolism, and related signaling pathways, and we can further assist with custom reagent development and assay optimization.